Pathway-specificity in N-methyl-d-aspartate receptor-mediated synaptic inputs onto subplate neurons
Pathway-specificity in N-methyl-d-aspartate receptor-mediated synaptic inputs onto subplate neurons
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N-甲基-d-天冬氨酸受体介导的亚板神经元突触输入的通路特异性
DOI：
10.1016/j.neuroscience.2008.01.068
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发表时间：
2008
期刊：
影响因子：
3.3
通讯作者：
H. J. Luhmann
中科院分区：
文献类型：
--
作者：
S. Hirsch;H. J. Luhmann
在早期皮层发育过程中，基底在神经元连接的形成中起着重要作用。我们使用全细胞和细胞贴附膜片钳记录新生小鼠（出生后[P] 0-3天）的冠状脑切片，表征了通往亚板神经元（SPn）、丘脑皮质和亚板内突触输入的两条主要通路的功能特性。细胞外分别用放置在丘脑和底板的双极电极刺激这两条传入通路。突触诱发和药理学分离的n -甲基-d-天冬氨酸受体（NMDAR）介导的反应，其发病潜伏期约为6 ms，可以在P0-3 SPn中可靠地记录下来。然而，在60-120毫秒的成对脉冲刺激或10-40赫兹的重复激活下，底板内输入显示出明显的促进作用，而在这些条件下，丘脑皮质输入要么稳定，要么明显受到抑制。单细胞反转录PCR结果显示，所有SPn均表达NR2A、B和D亚基。亚板内和丘脑皮质突触输入对NVP-AAM077或伊芬普罗地尔的敏感性没有差异，表明这两种突触输入具有相似的NR2A/2B亚基组成。在P0时，来自丘脑的nmdar介导的突触输入明显大于底板内输入。这种差异在P2-3 SPn中不再被检测到，这表明在第一个Ps中存在特定的输入发育调节。我们的数据表明，丘脑皮质和亚板内突触对P0-3 SPn的输入在功能、分子和发育特性上存在差异。亚板内突触输入显示出更成熟的功能特性，并维持较高的刺激频率，从而促进未成熟的丘脑皮层输入到发育中的新皮层回路。
The subplate plays an important role in forming neuronal connections during early cortical development. We characterized by the use of whole-cell and cell-attached patch-clamp recordings in coronal brain slices from newborn mice (postnatal day [P] 0–3) the functional properties of two major pathways onto subplate neurons (SPn), the thalamocortical and the intra-subplate synaptic input. The two afferent pathways were stimulated extracellularly with bipolar electrodes placed in the thalamus and the subplate, respectively. Synaptically evoked and pharmacologically isolated N-methyl-d-aspartate receptor (NMDAR) –mediated responses with an onset latency of ∼6 ms could be reliably recorded in P0-3 SPn. Whereas the intra-subplate input revealed a pronounced facilitation using paired pulse stimulation at 60–120 ms or repetitive activation at 10–40 Hz, the thalamocortical input was either stable or markedly suppressed under these conditions. Single cell reverse transcription PCR revealed the expression of the NR2A, B and D subunit in all investigated SPn. The intra-subplate and the thalamocortical synaptic input did not differ in their sensitivity to NVP-AAM077 or ifenprodil, indicating that both synaptic inputs have a similar NR2A/2B subunit composition. At P0, NMDAR-mediated synaptic inputs arising from the thalamus were significantly larger as compared with the intra-subplate input. This difference could no longer be detected in P2-3 SPn, indicating an input-specific developmental regulation during the first Ps. Our data indicate that the thalamocortical and intra-subplate synaptic input onto P0-3 SPn differs in functional, molecular and developmental properties. The intra-subplate synaptic input shows more mature functional properties and sustains high stimulation frequencies, thereby promoting the immature thalamocortical input to the developing neocortical circuit.
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影响因子：
3.6
作者：
K. Williams
通讯作者：
K. Williams
DOI：
10.1016/s0169-328x(99)00100-x
发表时间：
1999-06
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
D. Goebel;M. Poosch
通讯作者：
D. Goebel;M. Poosch
影响因子：
2.5
作者：
R. Tyzio;A. Ivanov;C. Bernard;G. Holmes;Y. Ben‐Ari;R. Khazipov
通讯作者：
R. Tyzio;A. Ivanov;C. Bernard;G. Holmes;Y. Ben‐Ari;R. Khazipov
影响因子：
56.9
作者：
CARMIGNOTO, G;VICINI, S
通讯作者：
VICINI, S
影响因子：
56.9
作者：
MCCONNELL, SK;GHOSH, A;SHATZ, CJ
通讯作者：
SHATZ, CJ