Modulation of plant DNA damage response gene expression during Agrobacterium infection.
Modulation of plant DNA damage response gene expression during Agrobacterium infection.
复制标题
农杆菌感染过程中植物 DNA 损伤反应基因表达的调节
DOI：
10.1016/j.bbrc.2021.03.044
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发表时间：
2021-05-21
影响因子：
3.1
通讯作者：
Citovsky V
中科院分区：
文献类型：
--
作者：
Hu Y;Lacroix B;Citovsky V
农杆菌T-DNA(Transfer DNA)整合到植物基因组中主要依赖于参与DNA损伤修复途径的宿主蛋白。然而，利用这些途径中涉及的突变或下调基因的植物获得了相互矛盾的结果。在这里，我们选择了一种不同的方法，通过跟踪一系列基因的表达，编码参与DNA损伤反应的蛋白质，在烟草农杆菌感染的早期阶段。首先，我们鉴定了DNA损伤诱导的拟南芥基因在烟草中的同源物，并证明它们的表达可以被DNA断裂诱导剂博莱霉素激活。然后，我们发现农杆菌感染诱导宿主DNA损伤反应的这些基因标记物中的几个表达，具有不同的转录反应模式。这种诱导在很大程度上依赖于农杆菌毒力因子，而不是T-DNA，这表明DNA损伤反应的激活可能依赖于农杆菌编码的毒力蛋白。我们的结果表明，农杆菌调节植物DNA损伤反应机制，这可能有助于细菌T-DNA整合到宿主基因组的DNA断裂中。
Agrobacterium T-DNA (transfer DNA) integration into the plant genome relies mostly on host proteins involved in the DNA damage repair pathways. However, conflicting results have been obtained using plants with mutated or down-regulated genes involved in these pathways. Here, we chose a different approach by following the expression of a series of genes, encoding proteins involved in the DNA damage response, during early stages of Agrobacterium infection in tobacco. First, we identified tobacco homologs of Arabidopsis genes induced upon DNA damage and demonstrated that their expression was activated by bleomycin, a DNA-break causing agent. Then, we showed that Agrobacterium infection induces the expression of several of these genes markers of the host DNA damage response, with different patterns of transcriptional response. This induction largely depends on Agrobacterium virulence factors, but not on the T-DNA, suggesting that the DNA damage response activation may rely on Agrobacterium-encoded virulence proteins. Our results suggest that Agrobacterium modulates the plant DNA damage response machinery, which might facilitate the integration of the bacterial T-DNA into the DNA breaks in the host genome.
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影响因子：
3.7
作者：
Baek, Eseul;Yoon, Ju-Yeon;Palukaitis, Peter
通讯作者：
Palukaitis, Peter
DOI：
10.1007/bf00331014
发表时间：
1986-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
KONCZ, C;SCHELL, J
通讯作者：
SCHELL, J
影响因子：
6.7
作者：
Song J;Bent AF
通讯作者：
Bent AF
影响因子：
10.5
作者：
GHEYSEN, G;VILLARROEL, R;VANMONTAGU, M
通讯作者：
VANMONTAGU, M
影响因子：
3.7
作者：
Ricaud L;Proux C;Renou JP;Pichon O;Fochesato S;Ortet P;Montané MH
通讯作者：
Montané MH