Secondary Mineralization of Ferrihydrite Affects Microbial Methanogenesis in Geobacter-Methanosarcina Cocultures
Secondary Mineralization of Ferrihydrite Affects Microbial Methanogenesis in Geobacter-Methanosarcina Cocultures
复制标题
水铁矿的二次矿化影响地杆菌-甲烷八叠球菌共培养物中微生物的产甲烷作用
DOI：
10.1128/aem.01517-16
复制
发表时间：
2016-07
影响因子：
4.4
通讯作者：
Zhou Shungui
中科院分区：
文献类型：
--
作者：
Ma Jinlian;Tang Ziyang;Yu Zhen;Zhou Shungui
随着时间的推移，水合铁转化为稳定的氧化铁对许多金属和营养物质的生物地球化学循环具有重要影响。产甲烷活性对氧化铁存在的响应取决于铁矿物的类型，但铁矿物学变化对产甲烷的影响尚未表征。为了解决这些问题，我们构建了具有不同水合铁矿化途径的Geobacter和Methanosarcina菌株的产甲烷共培养物。在该体系中，水合铁的二次矿化产物受磷酸盐的存在或不存在的调节。在产生磁铁矿作为二次矿化产物的培养基中，与不含水合铁的对照相比，乙酸和乙醇的产甲烷率分别提高了30.2%和135.3%。生物磁铁矿被认为可以促进Geobacter和Methanosarcina之间以类似于c型细胞色素的方式进行直接的种间电子转移，从而促进甲烷生成。在磷酸盐存在的情况下，铁水合石的维氏生物矿化对甲烷生成过程没有显著影响。在确定的共培养中添加磁铁矿后，乙酸和乙醇的甲烷产量增加，从而支持了磁铁矿发生与促进甲烷生成之间的相关性。我们的数据为不同厌氧环境下铁生物矿化在碳的生物地球化学循环中的重要作用提供了新的视角。研究发现，微生物产甲烷受铁矿物存在的影响，其对产甲烷的影响与铁矿物的矿物学性质有关。然而，铁矿物学的变化如何影响微生物产甲烷还没有被表征。为了解决这个问题，我们构建了具有不同水合铁矿化途径的Geobacter和Methanosarcina菌株的产甲烷共培养物。实验结果有两个贡献，即：(i)铁矿物的转化可能对厌氧条件下的甲烷生成产生重要影响；（ii）生物和化学磁铁矿都可以加速金属还原Geobacter metallireducens和Methanosarcina barkeri之间的合成乙醇氧化。该研究揭示了铁生物矿化在不同厌氧环境中，特别是在富铁的自然和农业湿地土壤中碳的生物地球化学循环中的重要作用。
ABSTRACT The transformation of ferrihydrite to stable iron oxides over time has important consequences for biogeochemical cycling of many metals and nutrients. The response of methanogenic activity to the presence of iron oxides depends on the type of iron mineral, but the effects of changes in iron mineralogy on methanogenesis have not been characterized. To address these issues, we constructed methanogenic cocultures of Geobacter and Methanosarcina strains with different ferrihydrite mineralization pathways. In this system, secondary mineralization products from ferrihydrite are regulated by the presence or absence of phosphate. In cultures producing magnetite as the secondary mineralization product, the rates of methanogenesis from acetate and ethanol increased by 30.2% and 135.3%, respectively, compared with a control lacking ferrihydrite. Biogenic magnetite was proposed to promote direct interspecies electron transfer between Geobacter and Methanosarcina in a manner similar to that of c-type cytochrome and thus facilitate methanogenesis. Vivianite biomineralization from ferrihydrite in the presence of phosphate did not significantly influence the methanogenesis processes. The correlation between magnetite occurrence and facilitated methanogenesis was supported by increased rates of methane production from acetate and ethanol with magnetite supplementation in the defined cocultures. Our data provide a new perspective on the important role of iron biomineralization in biogeochemical cycling of carbon in diverse anaerobic environments. IMPORTANCE It has been found that microbial methanogenesis is affected by the presence of iron minerals, and their influences on methanogenesis are associated with the mineralogical properties of the iron minerals. However, how changes in iron mineralogy affect microbial methanogenesis has not been characterized. To address this issue, we constructed methanogenic cocultures of Geobacter and Methanosarcina strains with different ferrihydrite mineralization pathways. The experimental results led to two contributions, i.e., (i) the transformation of iron minerals might exert an important influence on methanogenesis under anaerobic conditions and (ii) both biogenic and chemical magnetite can accelerate syntrophic ethanol oxidization between Geobacter metallireducens and Methanosarcina barkeri. This study sheds new light on the important role of iron biomineralization in the biogeochemical cycling of carbon in diverse anaerobic environments, particularly in iron-rich natural and agricultural wetland soils.
登录
查看更多内容
影响因子：
2.2
作者：
Gálvez, N;Barrón, V;Torrent, J
通讯作者：
Torrent, J
影响因子：
2.8
作者：
D. Lovley;Robert T. Anderson
通讯作者：
D. Lovley;Robert T. Anderson
影响因子：
5
作者：
Kukkadapu, RK;Zachara, JM;Kennedy, DW
通讯作者：
Kennedy, DW
影响因子：
11.4
作者：
E. Muehe;M. Obst;A. Hitchcock;T. Tyliszczak;S. Behrens;C. Schröder;James M. Byrne;F. Marc Michel-F.-Marc-M
通讯作者：
E. Muehe;M. Obst;A. Hitchcock;T. Tyliszczak;S. Behrens;C. Schröder;James M. Byrne;F. Marc Michel-F.-Marc-M
影响因子：
4.4
作者：
Rotaru, Amelia-Elena;Shrestha, Pravin Malla;Lovley, Derek R.
通讯作者：
Lovley, Derek R.