Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence.
Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence.
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DOI：
10.1186/1477-7827-9-45
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发表时间：
2011-04-11
期刊：
影响因子：
--
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Matsuda Y;Ito Y;Hashimoto H;Yokoi H;Suzuki T
可以从泡眼金鱼的眼囊中收集大量的淋巴。我们尝试在泡眼金鱼的眼囊淋巴中诱导卵黄蛋白原(VTG)，并建立了一种用FITC标记斑马鱼卵母细胞VTG掺入的方法。雌激素能有效地诱导金鱼眼囊淋巴中的VTG。制备FITC标记的VTG后，将其注射到成熟雌性斑马鱼体内。在体内和体外实验中，检测了斑马鱼卵母细胞对FITC标记的VTG的掺入。注射FITC标记的VTG的斑马鱼雌性胚胎从卵黄囊发出FITC荧光，发育正常。该方法可用于斑马鱼卵母细胞的发育和内分泌相关实验。
Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling. Estrogen efficiently induced Vtg in the eye-sac lymph of goldfish. After FITC-labeled Vtg was prepared, it was injected into mature female zebrafish. Incorporation of FITC-labeled Vtg by zebrafish oocytes was detected in in vivo and in vitro experiments. The embryos obtained from zebrafish females injected with FITC-labeled Vtg emitted FITC fluorescence from the yolk sac and developed normally. This method for achieving Vtg incorporation by zebrafish oocytes could be useful in experiments related to the development and endocrinology of zebrafish oocytes.
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影响因子：
2.7
作者：
TYLER, CR;SUMPTER, JP;SWANSON, P
通讯作者：
SWANSON, P
影响因子：
0.9
作者：
Sawatari, Etsuko;Hashimoto, Hisashi;Wakamatsu, Yuko
通讯作者：
Wakamatsu, Yuko
影响因子：
2.7
作者：
LEGUELLEC, K;LAWLESS, K;TENNISWOOD, M
通讯作者：
TENNISWOOD, M
影响因子：
64.8
作者：
LAEMMLI, UK
通讯作者：
LAEMMLI, UK
DOI：
10.1016/s1095-6433(02)00348-3
发表时间：
2003-03-01
影响因子：
2.3
作者：
Fukada, H;Fujiwara, Y;Hara, A
通讯作者：
Hara, A