Centrosome attachment to the C. elegans male pronucleus is dependent on the surface area of the nuclear envelope.
Centrosome attachment to the C. elegans male pronucleus is dependent on the surface area of the nuclear envelope.
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DOI：
10.1016/j.ydbio.2008.12.030
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发表时间：
2009-03-15
影响因子：
2.7
通讯作者：
Starr, Daniel A.
中科院分区：
文献类型：
--
作者：
Meyerzon, Marina;Gao, Zhizhen;Liu, Jin;Wu, Jui-Ching;Malone, Christian J.;Starr, Daniel A.
关键词：
在原核迁移过程中，雄性原核和中心体之间必须保持密切的联系。在线虫中，内核膜LEM蛋白EMR-1和LEM-2的同时缺失，核膜蛋白LMN-1或BAF-1的缺失，或者核输入成分的缺失，都会导致小原核的胚胎死亡。在此，描述了线虫受精卵中一种新的中心体脱离表型。核膜有缺陷的受精卵具有小的原核，其中一个中心体与雄性原核分离。ZYG-12、SUN-1和LIS-1与强力素一起作用于核膜以附着中心小体，在正常浓度下观察到它们位于具有分离中心小体的原核的核膜上。对延时图像的分析表明，当突变的原核在表面积生长时，它们捕获了分离的中心体。较大的四倍体或较小的组蛋白：：m樱桃原核分别抑制或增强中心体脱离表型。在用无核精子受精的胚胎中，只有一个中心体被小的雌性原核捕获，这表明捕获的机制取决于可与星形微管相互作用的外核膜的表面积。我们认为中心体附着在异常小原核表面的限制因素是动力蛋白。
A close association must be maintained between the male pronucleus and the centrosomes during pronuclear migration. In C. elegans, simultaneous depletion of inner nuclear membrane LEM proteins EMR-1 and LEM-2, depletion of the nuclear lamina proteins LMN-1 or BAF-1, or the depletion of nuclear import components leads to embryonic lethality with small pronuclei. Here, a novel centrosome detachment phenotype in C. elegans zygotes is described. Zygotes with defects in the nuclear envelope had small pronuclei with a single centrosome detached from the male pronucleus. ZYG-12, SUN-1, and LIS-1, which function at the nuclear envelope with dynein to attach centrosomes, were observed at normal concentrations on the nuclear envelope of pronuclei with detached centrosomes. Analysis of time-lapse images showed that as mutant pronuclei grew in surface area, they captured detached centrosomes. Larger tetraploid or smaller histone::mCherry pronuclei suppressed or enhanced the centrosome detachment phenotype respectively. In embryos fertilized with anucleated sperm, only one centrosome was captured by small female pronuclei, suggesting the mechanism of capture is dependent on the surface area of the outer nuclear membrane available to interact with aster microtubules. We propose that the limiting factor for centrosome attachment to the surface of abnormally small pronuclei is dynein.
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影响因子：
2.7
作者：
CARRE, D;ROUVIERE, C;SARDET, C
通讯作者：
SARDET, C
影响因子：
3.3
作者：
Askjaer, P;Galy, V;Mattaj, IW
通讯作者：
Mattaj, IW
影响因子：
4
作者：
Cockell, MM;Baumer, M;Gönczy, P
通讯作者：
Gönczy, P
影响因子：
64.8
作者：
BORNENS, M
通讯作者：
BORNENS, M
DOI：
10.1073/pnas.0307880101
发表时间：
2004-05-04
影响因子：
11.1
作者：
Fridkin, A;Mills, E;Gruenbaum, Y
通讯作者：
Gruenbaum, Y