Phylogenetic relatedness of circulating HIV-1C variants in Mochudi, Botswana.
Phylogenetic relatedness of circulating HIV-1C variants in Mochudi, Botswana.
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DOI：
10.1371/journal.pone.0080589
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Essex M
中科院分区：
文献类型：
--
作者：
Novitsky V;Bussmann H;Logan A;Moyo S;van Widenfelt E;Okui L;Mmalane M;Baca J;Buck L;Phillips E;Tim D;McLane MF;Lei Q;Wang R;Makhema J;Lockman S;DeGruttola V;Essex M
Determining patterns of HIV transmission is increasingly important for the most efficient use of modern prevention interventions. HIV phylogeny can provide a better understanding of the mechanisms underlying HIV transmission networks in communities. To reconstruct the structure and dynamics of a local HIV/AIDS epidemic, the phylogenetic relatedness of HIV-1 subtype C env sequences obtained from 785 HIV-infected community residents in the northeastern sector of Mochudi, Botswana, during 2010–2013 was estimated. The genotyping coverage was estimated at 44%. Clusters were defined based on relatedness of HIV-1C env sequences and bootstrap support of splits. The overall proportion of clustered HIV-1C env sequences was 19.1% (95% CI 17.5% to 20.8%). The proportion of clustered sequences from Mochudi was significantly higher than the proportion of non-Mochudi sequences that clustered, 27.0% vs. 14.7% (p = 5.8E-12; Fisher exact test). The majority of clustered Mochudi sequences (90.1%; 95% CI 85.1% to 93.6%) were found in the Mochudi-unique clusters. None of the sequences from Mochudi clustered with any of the 1,244 non-Botswana HIV-1C sequences. At least 83 distinct HIV-1C variants, or chains of HIV transmission, in Mochudi were enumerated, and their sequence signatures were reconstructed. Seven of 20 genotyped seroconverters were found in 7 distinct clusters. The study provides essential characteristics of the HIV transmission network in a community in Botswana, suggests the importance of high sampling coverage, and highlights the need for broad HIV genotyping to determine the spread of community-unique and community-mixed viral variants circulating in local epidemics. The proposed methodology of cluster analysis enumerates circulating HIV variants and can work well for surveillance of HIV transmission networks. HIV genotyping at the community level can help to optimize and balance HIV prevention strategies in trials and combined intervention packages.
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DOI：
10.1097/qad.0b013e3283121c90
发表时间：
2008-11-30
期刊：
AIDS (London, England)
影响因子：
--
作者：
Brenner BG;Roger M;Moisi DD;Oliveira M;Hardy I;Turgel R;Charest H;Routy JP;Wainberg MA;Montreal PHI Cohort and HIV Prevention Study Groups
通讯作者：
Montreal PHI Cohort and HIV Prevention Study Groups
影响因子：
6.4
作者：
Brenner, Bluma G.;Roger, Michel;Wainberg, Mark A.
通讯作者：
Wainberg, Mark A.
DOI：
10.1097/qad.0b013e3283573244
发表时间：
2012-09-10
期刊：
AIDS (London, England)
影响因子：
--
作者：
Dennis AM;Hué S;Hurt CB;Napravnik S;Sebastian J;Pillay D;Eron JJ
通讯作者：
Eron JJ
影响因子：
1.5
作者：
Cho, Young-Keol;Jung, You-Sun;Foley, Brian T.
通讯作者：
Foley, Brian T.
影响因子：
3.3
作者：
Bello, Gonzalo;Aulicino, Paula C.;Morgado, Mariza G.
通讯作者：
Morgado, Mariza G.