Hepatitis B virus DNA in liver and white blood cells of patients with hepatoma.
Hepatitis B virus DNA in liver and white blood cells of patients with hepatoma.
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肝癌患者肝脏和白细胞中的乙型肝炎病毒 DNA。
DOI：
10.1089/dna.1983.2.301
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
A. R. Herrera
中科院分区：
文献类型：
--
作者：
L. Lie;M. Balasegaram;C. Lopez;A. R. Herrera
用克隆于pBR325中的32P标记的HBVDNA进行Southern杂交分析，对23例原发性肝细胞癌患者的肝DNA和其中11例患者的白细胞DNA中的HBVDNA进行研究。在23例肝癌DNA中，16例HBVDNA阳性，其中15例显示HBVDNA整合到宿主肝DNA中。在5例患者中，除整合的HBVDNA外，还发现游离的HBVDNA，仅有1例单独发现游离的HBVDNA。所有血清乙肝表面抗原(HBs)阳性的患者肿瘤标本中HBVDNA均为阳性。不同病例的杂交方式和杂交程度有较大差异。2例肿瘤组织及癌旁组织中均检出HBVDNA。在11份白细胞DNA样本中，有两份为HBVDNA阳性。白细胞中的HBVDNA未整合到宿主DNA中。在未消化的白细胞DNA中，游离的HBVDNA在5.5kb处出现阳性信号，通常也在9.5kb处出现阳性信号。经Eco RI双酶切后，5.5kb和9.5kb的阳性片段消失，3.2-kb处出现一条强阳性条带。Hind III消化产生与未消化的白细胞DNA相同的阳性片段，但未能产生3.2kb的片段。有时，特别是在Hind III酶切后，除了5.5kb和9.5kb的阳性片段外，还可以在约16.2kb的位置显示出阳性片段。
Hepatitis B virus (HBV) DNA was studied in liver DNA of 23 patients with primary hepatocellular carcinoma and in white blood cell DNA of 11 of these patients by Southern blot hybridization analysis probed with 32P-labeled HBV DNA cloned in plasmid pBR325. Of the 23 hepatoma DNA samples, 16 were positive for HBV DNA, and 15 of these showed integration of HBV DNA into the host liver DNA. In 5 patients, free HBV DNA was found in addition to integrated HBV DNA and in only one was free HBV found alone. All patients serologically positive for HBV surface antigen (HBsAg) were positive for HBV DNA in tumor samples. The pattern and the degree of hybridization differed considerably among different cases. HBV DNA was found in tumor and in adjacent nontumor tissue in two patients. Of 11 white blood cell DNA samples, two were positive for HBV DNA. The HBV DNA in the white blood cells was not integrated into the host DNA. In the undigested white blood cell DNA, the free HBV DNA gave a positive signal at 5.5 kb and often also at 9.5 kb. After Eco RI digestion, these 5.5-kb and 9.5-kb positive fragments disappeared, while a strong positive band at 3.2-kb appeared. Hind III digestion produced the same positive fragments as in the undigested white blood cell DNA and failed to produce the 3.2-kb fragment. Sometimes, especially after Hind III digestion, a positive fragment at a position corresponding to about 16.2 kb was also demonstrable in addition to the 5.5-kb and 9.5-kb positive fragments.(ABSTRACT TRUNCATED AT 250 WORDS)
影响因子：
158.5
作者：
SHAFRITZ, DA;SHOUVAL, D;KEW, MC
通讯作者：
KEW, MC