Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes.
Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes.
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DOI：
10.3109/09553002.2012.702297
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发表时间：
2012-12
影响因子：
2.6
通讯作者：
Kassis AI
中科院分区：
文献类型：
--
作者：
Mamlouk O;Balagurumoorthy P;Wang K;Adelstein SJ;Kassis AI
目的研究DNA掺入125 I标记的人淋巴细胞对体外共培养的人结肠腺癌LS 174 T细胞的旁观者效应。用植物血凝素（PHA）刺激人外周血淋巴细胞合成DNA，并用5-[125 I]碘-2 ′-脱氧尿苷标记。将人结肠腺癌LS 174 T细胞与125 I标记的淋巴细胞以各种比例共培养5天，并评估LS 174 T细胞的增殖。此外，将来自这些共培养物的上清液培养基：（i）转移至LS 174 T细胞并在5天后测量其增殖，（ii）用于评估LS 174 T细胞的克隆形成存活，和（iii）筛选抑制生长的因子。当与125 I标记的淋巴细胞（56 ± 3.5%）或从这些共培养物获得的上清液培养基（52.5 ± 1.3%）共培养时，观察到LS 174 T细胞的增殖显著降低。在上清液培养基中生长的LS 174 T细胞的克隆形成存活证实了肿瘤细胞生长的减少。在存在125 I标记的淋巴细胞或从这种共培养物获得的培养基的情况下观察到的LS 174 T细胞增殖的减少可归因于抑制性（抗增殖）旁观者效应，其可能由死亡的125 I标记的淋巴细胞释放的因子介导。
To investigate the ability of human lymphocytes labeled with DNA-incorporated 125I to exert an inhibitory (antiproliferative) bystander effect on co-cultured human colon adenocarcinoma LS174T cells in vitro. Human peripheral blood lymphocytes were stimulated to synthesize DNA in the presence of phytohemagglutinin (PHA) and labeled with 5-[125I]iodo-2′-deoxyuridine. Human colon adenocarcinoma LS174T cells were co-cultured with the 125I-labeled lymphocytes in various ratios for 5 days and the proliferation of the LS174T cells was assessed. Further, the supernatant media from these co-cultures were: (i) Transferred to LS174T cells and their proliferation measured after 5 days, (ii) used to assess the clonogenic survival of LS174T cells, and (iii) screened for factors that suppress growth. A significant reduction in the proliferation of LS174T cells was observed when co-cultured either with 125I-labeled lymphocytes (56 ± 3.5%) or the supernatant media (52.5 ± 1.3%) obtained from these co-cultures. Clonogenic survival of LS174T cells grown in the supernatant media corroborated the decrease in tumor cell growth. The observed reduction in the proliferation of LS174T cells in presence of 125I-labeled lymphocytes or media obtained from such co-cultures can be attributed to an inhibitory (antiproliferative) bystander effect, probably mediated by factor(s) released from the dying 125I-labeled lymphocytes.
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影响因子：
3.3
作者：
Boyd, Marie;Sorensen, Annette;Mairs, Robert J.
通讯作者：
Mairs, Robert J.
影响因子：
3.4
作者：
Chang, CC;Sun, W;Trosko, JE
通讯作者：
Trosko, JE
DOI：
10.1073/pnas.011417098
发表时间：
2001-01-16
影响因子：
11.1
作者：
Azzam, EI;de Toledo, SM;Little, JB
通讯作者：
Little, JB
影响因子：
3.4
作者：
Anderson, CW;Dunn, JJ;Allalunis-Turner, MJ
通讯作者：
Allalunis-Turner, MJ
影响因子：
2.6
作者：
Frankenberg, D;Greif, KD;Giesen, U
通讯作者：
Giesen, U