Isolation of UV-resistant revertants from a xeroderma pigmentosum complementation group A cell line
Isolation of UV-resistant revertants from a xeroderma pigmentosum complementation group A cell line
复制标题
从着色性干皮病互补 A 组细胞系中分离抗紫外线回复体
作者：
B. Royer;W. Haseltine
着色性干皮病 (XP) 是一种常染色体隐性遗传疾病。 XP 患者培养的细胞对紫外线的致命作用非常敏感1-6。大多数 XP 细胞在紫外线引起的损伤的 DNA 修复的早期阶段存在缺陷。 XP 综合征遗传缺陷的性质尚未明确。为了解决这个问题，我们尝试从 XP 补充 A 组 (XPA) 患者的细胞系中分离出抗紫外线细胞。通过使用能够在 108 个细胞群中检测一个抗紫外线细胞的选择方案，以 1 × 10−7 和 2 × 10−8 之间的频率分离出几个抗紫外线克隆。在这里，我们描述了这些表型回复突变体的分离和初步表征。
Xeroderma pigmentosum (XP) is an autosomal recessive disease. Cells cultured from XP patients are hypersensitive to the lethal effects of UV light1–6. Most XP cells are defective in an early stage in DNA repair of UV light-induced damage. The nature of the genetic defect of the XP syndrome has not been defined. To address this problem, we attempted to isolate UV-resistant cells from a cell line derived from an XP complementation group A (XPA) patient. By using a selection scheme capable of detecting one UV-resistant cell in a population of 108 cells, several UV-resistant clones were isolated at frequencies between 1 × 10−7 and 2 × 10−8. Here we describe the isolation and initial characterization of these phenotypic revertants.
通过碱性琼脂糖凝胶电泳定量非放射性脱氧核糖核酸的嘧啶二聚体含量。
影响因子：
2.9
作者：
Sutherland,BM;Shih,AG
通讯作者：
Shih,AG