Effect and mechanism of poly (ADP-ribose) polymerase-1 in aldosterone-induced apoptosis.
Effect and mechanism of poly (ADP-ribose) polymerase-1 in aldosterone-induced apoptosis.
复制标题
聚(ADP-核糖)聚合酶1在醛固酮诱导细胞凋亡中的作用及机制
DOI：
10.3892/mmr.2015.3596
复制
发表时间：
2015-08
影响因子：
3.4
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Qiao W;Zhang W;Shao S;Gai Y;Zhang M
本研究旨在探讨醛固酮对血管内皮细胞的作用及对多聚腺苷二磷酸核糖聚合酶1（poly（ADP-ribose）polymerase 1，PARP 1）活性的影响，并探讨醛固酮抗血管内皮细胞损伤的分子机制。培养的内皮细胞分别用不同浓度的醛固酮作用相同时间或相同浓度的醛固酮作用不同时间，检测细胞凋亡水平和PARP 1活性。在醛固酮处理过程中加入醛固酮受体拮抗剂或PARP 1抑制剂，检测细胞凋亡水平和PARP 1活性。随着醛固酮浓度的增加或作用时间的延长，凋亡细胞数和PARP 1活性增加。醛固酮受体拮抗剂和PARP 1抑制剂可抑制醛固酮引起的细胞凋亡和PARP 1活性的增加。醛固酮通过醛固酮受体激活PARP 1活性，抑制细胞增殖并诱导细胞凋亡。PARP 1治疗可作为高浓度醛固酮引起的血管疾病的靶点。
The present study aimed to investigate the effects of aldosterone on vascular endothelial cells and the viability of poly (ADP-ribose) polymerase 1 (PARP1) in cells, and to examine the molecular mechanisms underlying the effects of aldosterone on vascular endothelial cell injury. Cultured endothelial cells were treated either with different concentrations of aldosterone for the same duration or with the same concentrations of aldosterone for different durations, and the levels of apoptosis and activity of PARP1 in the cells were detected, respectively. Aldosterone receptor antagonists or PARP1 inhibitors were added to cells during treatment with aldosterone and the levels of apoptosis and activity of PARP1 were detected. As the concentration of aldosterone increased or the treatment time increased, the number of apoptotic cells and the activity of PARP1 increased. The aldosterone receptor antagonists and PARP1 inhibitors inhibited the increase of apoptosis and PARP1 activity caused by aldosterone treatment. Aldosterone activated the activity of PARP1 via the aldosterone receptor, inhibiting cell proliferation and inducing apoptosis. Treatment with PARP1 may be used as a target for vascular diseases caused by aldosterone at high concentrations.
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影响因子：
37.8
作者：
Keidar, S;Kaplan, M;Aviram, M
通讯作者：
Aviram, M
影响因子：
37.3
作者：
Sand-Dejmek J;Adelmant G;Sobhian B;Calkins AS;Marto J;Iglehart DJ;Lazaro JB
通讯作者：
Lazaro JB
影响因子：
14.9
作者：
Schultz, N;Lopez, E;Helleday, T
通讯作者：
Helleday, T
肿瘤坏死因子样弱凋亡诱导剂和成纤维细胞生长因子诱导型 14 介导脑缺血诱导的聚（ADP-核糖）聚合酶-1 激活和神经元死亡。
影响因子：
3.3
作者：
Haile, W. B.;Echeverry, R.;Wu, F.;Guzman, J.;An, J.;Wu, J.;Yepes, M.
通讯作者：
Yepes, M.
影响因子：
8.3
作者：
Takai, S;Jin, D;Miyazaki, M
通讯作者：
Miyazaki, M