Inhibition of microRNA-92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy-related phenotype in mdx mice.
Inhibition of microRNA-92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy-related phenotype in mdx mice.
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DOI：
10.1002/mus.26433
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发表时间：
2019-05
期刊：
影响因子：
3.4
通讯作者：
Asakura A
中科院分区：
文献类型：
--
作者：
Verma M;Asakura Y;Asakura A
Duchenne型肌营养不良症（DMD）及其mdx小鼠模型肌肉血管和血流受到干扰。microRNA-92 a（miR-92 a）在内皮细胞中富集，尤其是在缺血性损伤期间。由于拮抗miR-92 a显示出导致内皮细胞的增殖和迁移增加以及从缺血中恢复，因此我们评估了Antagomir-92 a在肌肉干细胞培养物中的体外作用和在mdx小鼠中的体内作用。发现miR-92 a在肌肉内皮细胞和卫星细胞中高度表达。用Antagomir-92 a处理增加毛细血管密度和组织灌注，这伴随着卫星细胞的增加。然而，Antagomir-92 a处理的mdx小鼠没有显示出组织学改善，并且具有更差的肌肉功能。Antagomir-92 a抑制卫星细胞培养中的成肌分化。AntagomiR-92 a改善了mdx小鼠的血管系统，但没有改善肌肉，这可能是由于其对卫星细胞分化的副作用。
The vasculature and blood flow in muscle are perturbed in Duchenne muscular dystrophy (DMD) and its mdx mouse model. MicroRNA-92a (miR-92a) is enriched in endothelial cells, especially during ischemic injury. Because antagonizing miR-92a was shown to result in increased proliferation and migration of endothelial cells and recovery from ischemia, we assessed the effects of Antagomir-92a in vitro in muscle stem cell culture and in vivo in mdx mice. miR-92a was found to be highly expressed in muscle endothelial cells and satellite cells. Treatment with Antagomir-92a increased capillary density and tissue perfusion, which was accompanied by an increase in satellite cells. However, Antagomir-92a–treated mdx mice showed no histological improvement and had worse muscle function. Antagomir-92a suppressed myogenic differentiation in satellite cell culture. AntagomiR-92a improves the vasculature but not the muscle in mdx mice, possibly due to its side effects on satellite cell differentiation.
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影响因子：
12.4
作者：
通讯作者：
--
影响因子：
20.1
作者：
Loyer, Xavier;Potteaux, Stephane;Tedgui, Alain
通讯作者：
Tedgui, Alain
DOI：
10.1073/pnas.86.4.1292
发表时间：
1989-02-01
影响因子：
11.1
作者：
CHAPMAN, VM;MILLER, DR;CASKEY, CT
通讯作者：
CASKEY, CT
DOI：
10.3791/50846
发表时间：
2014-04-08
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Motohashi N;Asakura Y;Asakura A
通讯作者：
Asakura A
影响因子：
5.5
作者：
Call, Jarrod A.;Warren, Gordon L.;Lowe, Dawn A.
通讯作者：
Lowe, Dawn A.