Technical Advances in the Measurement of Residual Disease in Acute Myeloid Leukemia.
Technical Advances in the Measurement of Residual Disease in Acute Myeloid Leukemia.
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DOI：
10.3390/jcm6090087
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发表时间：
2017-09-19
影响因子：
3.9
通讯作者：
Dillon LW
中科院分区：
文献类型：
--
作者：
Roloff GW;Lai C;Hourigan CS;Dillon LW
诊断为急性髓细胞白血病（AML）的患者的结局仍然很差。已广泛确定，诱导治疗后或造血干细胞移植（HSCT）时的持续残留白血病负荷（通常称为可测量或微小残留病（MRD））高度预测不良临床结局，并可用于识别可能发生临床明显复发的患者。由于AML中固有的遗传和分子异质性，没有统一的方法或方案用于MRD测量以涵盖所有病例。已经描述了几种专注于识别复发性分子和细胞遗传学畸变或白血病相关免疫表型的技术，每种技术都有自己的优点和缺点。能够对单个DNA或RNA分子进行数字量化和跟踪的现代技术、下一代测序（NGS）平台和高分辨率成像能力是正在开发的几种新途径之一，以补充或取代当前的流式细胞术标准。在这篇综述中，我们概述了新兴的方式定位，以提高MRD检测和讨论的因素，他们融入临床实践。
Outcomes for those diagnosed with acute myeloid leukemia (AML) remain poor. It has been widely established that persistent residual leukemic burden, often referred to as measurable or minimal residual disease (MRD), after induction therapy or at the time of hematopoietic stem cell transplant (HSCT) is highly predictive for adverse clinical outcomes and can be used to identify patients likely to experience clinically evident relapse. As a result of inherent genetic and molecular heterogeneity in AML, there is no uniform method or protocol for MRD measurement to encompass all cases. Several techniques focusing on identifying recurrent molecular and cytogenetic aberrations or leukemia-associated immunophenotypes have been described, each with their own strengths and weaknesses. Modern technologies enabling the digital quantification and tracking of individual DNA or RNA molecules, next-generation sequencing (NGS) platforms, and high-resolution imaging capabilities are among several new avenues under development to supplement or replace the current standard of flow cytometry. In this review, we outline emerging modalities positioned to enhance MRD detection and discuss factors surrounding their integration into clinical practice.
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影响因子：
45.3
作者：
Grimwade, David;Jovanovic, Jelena V.;Burnett, Alan K.
通讯作者：
Burnett, Alan K.
影响因子：
14.9
作者：
Hoffmann C;Minkah N;Leipzig J;Wang G;Arens MQ;Tebas P;Bushman FD
通讯作者：
Bushman FD
影响因子：
48
作者：
Hindson, Christopher M.;Chevillet, John R.;Briggs, Hilary A.;Gallichotte, Emily N.;Ruf, Ingrid K.;Hindson, Benjamin J.;Vessella, Robert L.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh
影响因子：
7.4
作者：
Hindson, Benjamin J.;Ness, Kevin D.;Masquelier, Donald A.;Belgrader, Phillip;Heredia, Nicholas J.;Makarewicz, Anthony J.;Bright, Isaac J.;Lucero, Michael Y.;Hiddessen, Amy L.;Legler, Tina C.;Kitano, Tyler K.;Hodel, Michael R.;Petersen, Jonathan F.;Wyatt, Paul W.;Steenblock, Erin R.;Shah, Pallavi H.;Bousse, Luc J.;Troup, Camille B.;Mellen, Jeffrey C.;Wittmann, Dean K.;Erndt, Nicholas G.;Cauley, Thomas H.;Koehler, Ryan T.;So, Austin P.;Dube, Simant;Rose, Klint A.;Montesclaros, Luz;Wang, Shenglong;Stumbo, David P.;Hodges, Shawn P.;Romine, Steven;Milanovich, Fred P.;White, Helen E.;Regan, John F.;Karlin-Neumann, George A.;Hindson, Christopher M.;Saxonov, Serge;Colston, Bill W.
通讯作者：
Colston, Bill W.
影响因子：
3.7
作者：
Dube S;Qin J;Ramakrishnan R
通讯作者：
Ramakrishnan R