Plasma-sensitive Escherichia coli mutants reveal plasma resistance mechanisms
Plasma-sensitive Escherichia coli mutants reveal plasma resistance mechanisms
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血浆敏感大肠杆菌突变体揭示血浆抗性机制
DOI：
10.1098/rsif.2018.0846
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发表时间：
2019
影响因子：
3.9
通讯作者：
J. E. Bandow
中科院分区：
文献类型：
--
作者：
M. Krewing;F. Jarzina;T. Dirks;Britta Schubert;J. Benedikt;J.-W. Lackmann;J. E. Bandow
非热大气压等离子体被研究作为对抗细菌感染的增强疗法。等离子体的强抗菌效果归因于反应物种、（V）UV辐射和电场的复杂混合物。抗生素的经验是，在作为药物引入时，病原体会产生耐药性并扩散。为了评估细菌对血浆产生耐药性的可能性，我们研究了使大肠杆菌在血浆应激中存活的内在保护机制。我们进行了全基因组筛选的单基因敲除突变体的E。coliand鉴定了87种对微尺度大气压等离子体射流的流出物超敏感的突变体。对于选定的基因（cysB，mntH，repandiscS），我们在互补研究中表明，血浆抗性可以恢复和增加超过野生型水平后，过表达。为了鉴定87个基因赋予抗性的血浆衍生组分，测试了突变体对单个应激物（过氧化氢、超氧化物、羟基自由基、臭氧、HOCl、过氧亚硝酸盐、NO·、亚硝酸盐、硝酸盐、HNO 3、酸应激、二酰胺、热应激和洗涤剂）的超敏反应。k-means++聚类揭示了大多数基因保护免受过氧化氢、超氧化物和/或一氧化氮。总之，单个细菌基因赋予对血浆的抗性，提供了对血浆抗菌机制的见解。
Non-thermal atmospheric pressure plasmas are investigated as augmenting therapy to combat bacterial infections. The strong antibacterial effects of plasmas are attributed to the complex mixture of reactive species, (V)UV radiation and electric fields. The experience with antibiotics is that upon their introduction as medicines, resistance occurs in pathogens and spreads. To assess the possibility of bacterial resistance developing against plasma, we investigated intrinsic protective mechanisms that allowEscherichia colito survive plasma stress. We performed a genome-wide screening of single-gene knockout mutants ofE. coliand identified 87 mutants that are hypersensitive to the effluent of a microscale atmospheric pressure plasma jet. For selected genes (cysB,mntH,repandiscS) we showed in complementation studies that plasma resistance can be restored and increased above wild-type levels upon over-expression. To identify plasma-derived components that the 87 genes confer resistance against, mutants were tested for hypersensitivity against individual stressors (hydrogen peroxide, superoxide, hydroxyl radicals, ozone, HOCl, peroxynitrite, NO•, nitrite, nitrate, HNO3, acid stress, diamide, heat stress and detergents). k-means++ clustering revealed that most genes protect from hydrogen peroxide, superoxide and/or nitric oxide. In conclusion, individual bacterial genes confer resistance against plasma providing insights into the antibacterial mechanisms of plasma.
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影响因子：
2.9
作者：
R. Viner;T. Williams;C. Schöneich
通讯作者：
R. Viner;T. Williams;C. Schöneich
影响因子：
13.8
作者：
Bell JC;Kowalczykowski SC
通讯作者：
Kowalczykowski SC
影响因子：
10.5
作者：
Mettert EL;Kiley PJ
通讯作者：
Kiley PJ
影响因子：
3.4
作者：
Hefny, Mohamed Mokhtar;Pattyn, Cedric;Benedikt, Jan
通讯作者：
Benedikt, Jan
λdg DNA 的断裂：每个分离的半分子的化学和遗传特征
DOI：
--
发表时间：
1964
期刊：
影响因子：
--
作者：
D. Hogness;J. Simmons
通讯作者：
J. Simmons