Evidence of vacuolar compartmentalization of arsenic in the hyperaccumulator Pteris vittata
Evidence of vacuolar compartmentalization of arsenic in the hyperaccumulator Pteris vittata
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超积累植物 Pteris vittata 中砷液泡区室化的证据
DOI：
10.1007/s11434-009-0675-4
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发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
Yang XueXi
中科院分区：
文献类型：
--
作者：
Chen Hui;He ZhenYan;Ma Mi;Xu WenZhong;Dai XiaoJing;Yang XueXi
蜈蚣草可以超积累砷（As）至其干重的1%而不显示任何毒性迹象，表明存在有效的植物内部解毒机制。虽然已知液泡区隔化在重金属解毒和耐受中起重要作用，但缺乏砷在这种超蓄积物中区隔化的直接证据。在这里，我们报道了As在维塔塔愈伤组织中的亚细胞定位。采用直接分离细胞壁、原生质体和液泡并测定砷浓度的方法，对暴露于0.2 mmol/L砷酸盐环境20 d的维塔塔（P. vittata）配子体诱导的愈伤组织进行研究。愈伤组织中约94%的砷存在于原生质体中，其中91%存在于液泡中，说明液泡是维塔草中砷的主要储存场所。此外，利用傅里叶变换红外光谱（FTIR）分析了液泡化学成分的变化。
Pteris vittata can hyperaccumulate arsenic (As) to >1% of its dry weight without showing any signs of toxicity, indicating the existence of effective plant-internal detoxification mechanisms. Although vacuolar compartmentalization is known to play an important role in heavy metal detoxification and tolerance, direct evidence of arsenic compartmentalization in this hyperaccumulator was lacking. Here we report the subcellular localization of As in the callus of P. vittata. The callus induced from gametophytes of P. vittata exposed to 0.2 mmol/L arsenate for 20 days were examined by directly isolating cell walls, protoplasts and vacuoles, and determining arsenic concentrations. Of the total As in the callus, about 94% was in the protoplast, and of that, 91% was present in the vacuoles, indicating that vacuoles are a major storage site for As in P. vittata. In addition, the changes in the chemical components of vacuoles were analyzed by Fourier transform infrared spectroscopy (FTIR).
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DOI：
10.2136/sssabookser3.3ed
发表时间：
1990
期刊：
--
影响因子：
--
作者：
R. Westerman
通讯作者：
R. Westerman
影响因子：
4.3
作者：
Shen, RF;Ma, JF;Iwashita, T
通讯作者：
Iwashita, T
影响因子：
9.4
作者：
Bleeker, PM;Schat, H;Ernst, WHO
通讯作者：
Ernst, WHO
影响因子：
3.8
作者：
G. Bolwell
通讯作者：
G. Bolwell
影响因子：
4.2
作者：
P. Shewry
通讯作者：
P. Shewry