Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases.
Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases.
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DOI：
10.1083/jcb.200909005
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发表时间：
2010-03-08
期刊：
影响因子：
--
通讯作者：
Swedlow JR
中科院分区：
文献类型：
--
作者：
Jaqaman K;King EM;Amaro AC;Winter JR;Dorn JF;Elliott HL;McHedlishvili N;McClelland SE;Porter IM;Posch M;Toso A;Danuser G;McAinsh AD;Meraldi P;Swedlow JR
一种自动化、定量的4D图像分析方法用于跟踪中期细胞的着丝点动力学。在大多数真核细胞的有丝分裂过程中，染色体排列并在纺锤极中间形成一个中期板，它们围绕着这个板表现出振荡运动。这些运动伴随着姐妹动点之间距离的变化，通常被称为呼吸。我们开发了一种结合计算图像分析的活细胞成像分析方法，在三维空间上量化姐妹动着点的特性和动力学。研究表明，前期晚期和中期的基线振荡和呼吸速度是由微管解聚合酶决定的，而振荡和呼吸周期则取决于姐妹之间机械连接的刚度。在一个相对恒定的振荡周期内，由于振荡速度降低，中期板随着细胞向后期发展而变薄。振动速度的逐渐放缓及其与板厚的耦合取决于姊妹间机械连杆的刚度。我们提出中期板的形成和变薄需要严格控制由着丝粒染色质和内聚介导的姐妹之间的机械连接状态。
An automated, quantitative 4D image analysis method is used to track kinetochore dynamics in metaphase cells. During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.
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DOI：
10.1016/j.cub.2009.04.036
发表时间：
2009-05-26
期刊：
Current biology : CB
影响因子：
--
作者：
Ke K;Cheng J;Hunt AJ
通讯作者：
Hunt AJ
影响因子：
7.8
作者：
Khodjakov, A;Cole, RW;McEwen, BF;Buttle, KF;Rieder, CL
通讯作者：
Rieder, CL
影响因子：
3.3
作者：
Kline-Smith, SL;Khodjakov, A;Walczak, CE
通讯作者：
Walczak, CE
影响因子：
16
作者：
Hunter, AW;Caplow, M;Howard, J
通讯作者：
Howard, J
影响因子：
9.2
作者：
Gerlich, Daniel;Koch, Birgit;Ellenberg, Jan
通讯作者：
Ellenberg, Jan