The distribution of polar ejection forces determines the amplitude of chromosome directional instability.
The distribution of polar ejection forces determines the amplitude of chromosome directional instability.
复制标题
DOI：
10.1016/j.cub.2009.04.036
复制
发表时间：
2009-05-26
期刊：
影响因子：
--
通讯作者：
Hunt AJ
中科院分区：
文献类型：
--
作者：
Ke K;Cheng J;Hunt AJ
极性抛射力（pef）通常被假设为指导有丝分裂染色体的定向不稳定性，但从未建立直接联系。这在一定程度上导致了另类理论的复兴。利用极其精确的飞秒脉冲激光显微手术，我们通过切断脊椎动物染色体臂来突然改变pef的大小。pef的减少增加了方向不稳定性的幅度，而不改变其他特征，从而在pef和染色体运动方向之间建立了直接联系。我们发现，pef通过增加前一个着丝点上的马达突然脱离或关闭的可能性，从而导致方向逆转，从而限制了染色体振荡的范围。根据振荡幅度的变化与染色体臂缩短的数量之间的关系，我们能够绘制出pef在纺锤体上的分布，这与先前假设的分布惊人地不同。这些结果使我们能够区分提出的机制之间的染色体的定向不稳定性的基础。
Polar ejection forces (PEFs) have often been hypothesized to guide directional instability of mitotic chromosomes, but a direct link has never been established. This has lead, in part, to the resurgence of alternative theories. Taking advantage of extremely precise femtosecond pulsed laser microsurgery, we abruptly alter the magnitude of PEFs by severing vertebrate chromosome arms. Reduction of PEFs increases the amplitude of directional instability without altering other characteristics, thus establishing a direct link between PEFs and the direction of chromosome movements. We find that PEFs limit the range of chromosome oscillations by increasing the probability that motors at a leading kinetochore abruptly disengage or turn off, leading to a direction reversal. From the relation between the change in oscillation amplitude and the amount the chromosome arm is shortened, we are able to map the distribution of PEFs across the spindle, which is surprisingly different from previously assumed distributions. These results allow us to differentiate between the mechanisms proposed to underlie the directional instability of chromosomes.
登录
查看更多内容
DOI：
10.1109/2944.796309
发表时间：
1999-07-01
影响因子：
4.9
作者：
Juhasz, T;Frieder, H;Mourou, G
通讯作者：
Mourou, G
影响因子：
7.8
作者：
Khodjakov, A;Rieder, CL
通讯作者：
Rieder, CL
影响因子：
7.4
作者：
Ke, K;Hasselbrink, EF;Hunt, AJ
通讯作者：
Hunt, AJ
DOI：
10.1083/jcb.109.2.637
发表时间：
1989-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mitchison TJ
通讯作者：
Mitchison TJ
影响因子：
3.3
作者：
Gardner, MK;Pearson, CG;Odde, DJ
通讯作者：
Odde, DJ