Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 2010.
Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 2010.
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DOI：
10.1371/journal.pone.0030946
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Nakaya T
中科院分区：
文献类型：
--
作者：
Yasugi M;Nakamura S;Daidoji T;Kawashita N;Ramadhany R;Yang CS;Yasunaga T;Iida T;Horii T;Ikuta K;Takahashi K;Nakaya T
2009年4月，出现了一种新型猪源甲型流感病毒（H1N1pdm），并迅速在包括日本在内的世界各地传播。有人认为，该病毒可以与受感染哺乳动物的 2,3- 和 2,6- 连接唾液酸受体结合，这与当代季节性 H1N1 病毒不同，后者偏爱 2,6- 连接唾液酸。为了阐明 H1N1pdm 中 α2,3 唾液酸特异性病毒的存在和传播性，研究了病毒血凝素分子内的氨基酸取代，特别是 D187E、D222G 和 Q223R，它们与从人类受体特异性到禽类受体特异性的转变有关。使用高通量测序方法对日本第一波和第二波疫情期间感染者的样本进行了检查。 2009 年 5 月，来自轻症病例的三份样本显示，感染这些个体的一小部分病毒存在 D222G 和/或 Q223R 替换。然而，2010 年 12 月 5 例轻症病例的样本中，这些替换几乎消失。即使在 2009 年 5 月，D187E 替换在样本中也并不普遍。这些结果表明，α2,3 唾液酸特异性病毒，包括 G222 和 R223，在大流行早期阶段作为少数群体存在于人类中，而 D222 和 Q223 在大流行期间通过人际传播变得更加占主导地位。 第一波和第二波疫情。这些结果与 2008 年季节性 H1N1 病毒中发现的低替代率一致。
In April 2009, a novel swine-derived influenza A virus (H1N1pdm) emerged and rapidly spread around the world, including Japan. It has been suggested that the virus can bind to both 2,3- and 2,6-linked sialic acid receptors in infected mammals, in contrast to contemporary seasonal H1N1 viruses, which have a predilection for 2,6-linked sialic acid. To elucidate the existence and transmissibility of α2,3 sialic acid-specific viruses in H1N1pdm, amino acid substitutions within viral hemagglutinin molecules were investigated, especially D187E, D222G, and Q223R, which are related to a shift from human to avian receptor specificity. Samples from individuals infected during the first and second waves of the outbreak in Japan were examined using a high-throughput sequencing approach. In May 2009, three specimens from mild cases showed D222G and/or Q223R substitutions in a minor subpopulation of viruses infecting these individuals. However, the substitutions almost disappeared in the samples from five mild cases in December 2010. The D187E substitution was not widespread in specimens, even in May 2009. These results suggest that α2,3 sialic acid-specific viruses, including G222 and R223, existed in humans as a minor population in the early phase of the pandemic, and that D222 and Q223 became more dominant through human-to-human transmission during the first and second waves of the epidemic. These results are consistent with the low substitution rates identified in seasonal H1N1 viruses in 2008.
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影响因子：
11.8
作者：
Nakamura S;Maeda N;Miron IM;Yoh M;Izutsu K;Kataoka C;Honda T;Yasunaga T;Nakaya T;Kawai J;Hayashizaki Y;Horii T;Iida T
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