Expression of myelin genes: comparative analysis of Oli-neu and N20.1 oligodendroglial cell lines.
Expression of myelin genes: comparative analysis of Oli-neu and N20.1 oligodendroglial cell lines.
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DOI：
10.1002/jnr.22625
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发表时间：
2011-07
影响因子：
4.2
通讯作者：
Wight, Patricia A.
中科院分区：
文献类型：
--
作者：
Pereira, Glauber B.;Dobretsova, Anna;Hamdan, Hamdan;Wight, Patricia A.
永生化细胞的使用已经成为研究基因调控的工具。然而，了解特定细胞系的状态是至关重要的，特别是在研究其表达受发育调节的基因的调控时。从小鼠少突胶质细胞原代培养中获得了几种永生化细胞系。两个这样的细胞系- N20.1和Oli-neu -在mRNA和蛋白水平上的髓磷脂基因的相对表达进行了表征。对髓磷脂蛋白脂质蛋白（Plp1）、髓磷脂碱性蛋白（Mbp）和2 ',3 ' -环核苷酸3 ' -磷酸二酯酶（Cnp）基因表达的剪接异构体以及这些基因表达的相对蛋白量的分析表明，这些细胞系是未成熟少突胶质细胞的代表，尽管与N20.1细胞相比，Oli-neu细胞似乎在分化途径上走得更远。先前的研究表明，在髓鞘形成活跃期Plp1基因表达的发育增加是由第一个内含子内的转录调控元件控制的。在两种细胞系中研究了其中一种元素——所谓的抗沉默/增强（ASE）——的反应性。本研究的结果表明，ASE在oli - new细胞中具有更强的作用。因此，这两种细胞系似乎处于不同的阶段，将有助于研究在发育过程中影响变化的转录调控元件。
The use of immortalized cells has been instrumental as a tool in which to study gene regulation. However it is crucial to understand the status of a given cell line, especially when investigating the regulation of genes whose expression is developmentally regulated. Several immortalized cell lines have been derived from primary cultures of mouse oligodendrocytes. Two such cell lines – N20.1 and Oli-neu – were characterized here in terms of their relative expression of myelin genes at both the mRNA and protein level. Analysis of the splice isoforms expressed by the myelin proteolipid protein (Plp1), myelin basic protein (Mbp), and 2’,3’-cyclic nucleotide 3’-phosphodiesterase (Cnp) genes, along with the relative amount of protein expressed by these genes suggest that the cell lines are representative of immature oligodendrocytes, although Oli-neu cells appear to be further along the differentiation pathway compared to N20.1 cells. Previous studies have shown that the developmental increase in Plp1 gene expression that occurs during the active myelination period is governed by transcription regulatory elements present within the first intron. The responsiveness of one of these elements – the so-called antisilencer/enhancer (ASE) – was investigated in both cell lines. Results presented here suggest that the ASE has a much more potent effect in Oli-neu cells. Thus, the two cell lines appear to be at different stages and will be useful as a means to study transcription regulatory elements whose influence changes during development.
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影响因子：
4.7
作者：
Dobretsova, Anna;Johnson, Jennifer W.;Wight, Patricia A.
通讯作者：
Wight, Patricia A.
影响因子：
4.7
作者：
Dobretsova, A;Wight, PA
通讯作者：
Wight, PA
影响因子：
5.3
作者：
GARDINIER, MV;MACKLIN, WB;DEININGER, PL
通讯作者：
DEININGER, PL
影响因子：
4.2
作者：
Meng, FX;Zolova, O;Wight, PA
通讯作者：
Wight, PA
影响因子：
4.7
作者：
VERITY, AN;BREDESEN, D;CAMPAGNONI, AT
通讯作者：
CAMPAGNONI, AT