Binding of both acrosome-intact and acrosome-reacted guinea pig sperm to the zona pellucida during in vitro fertilization.
Binding of both acrosome-intact and acrosome-reacted guinea pig sperm to the zona pellucida during in vitro fertilization.
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在体外受精过程中，顶体完整和顶体反应的豚鼠精子与透明带的结合。
DOI：
10.1016/0012-1606(87)90191-6
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发表时间：
1987
影响因子：
2.7
通讯作者：
P. Primakoff
中科院分区：
文献类型：
--
作者：
D. Myles;H. Hyatt;P. Primakoff
当精子与卵子的透明带结合时，哺乳动物的精子-卵子就会发生粘连。在这项以豚鼠配子为对象的研究中，我们询问了精子在顶体反应前后是否可以启动与顶体的结合，以及精子表面蛋白PH-20是否参与了这两个阶段的结合。在各种条件下对精子与卵泡的结合进行了检测。精子悬浮在0.9%的氯化钠中，或用两种不同的方法获能。卵子被固定在涂有凝集素的盖片上，在盖片和玻璃片之间挤压，或者在组织培养皿中自由放置。精子-卵子的相互作用被记录在录像带上或在将卵子与结合的精子固定后进行评估。在所研究的所有条件下，顶体完整的和顶体反应的精子都开始与小带结合。这种结合是持久的，不是暂时的。特别是，观察到与小带结合的顶体完整的精子保持结合长达80分钟。一个顶体完整的精子与顶体结合在一起，在顶体发生反应时被录像。当混合精子群(平均24%的顶体完整和76%的顶体反应)与卵子孵育30min时，平均有10%的结合精子是顶体完整的。PH-20单抗先前已被证明可抑制未确定顶体状态的豚鼠精子与透明带的结合(P.Primakoff，H.Hyatt，and D.G.Myles(1985)，J.Cell Biol.101,2239-2244)。在这项研究中，当两组精子分别计数时，PH-20抑制顶体反应的精子的结合，但不抑制顶体完整的精子。我们的结果表明，顶体完整的和顶体反应的豚鼠精子都可以启动与顶体的结合；然而，这两种情况下的结合可能不是通过相同的机制发生的。
Mammalian sperm-egg adhesion occurs when sperm bind to the zona pellucida of the egg. In this study with guinea pig gametes, we have asked if sperm can initiate binding to the zona before and after the acrosome reaction and if the sperm surface protein PH-20 is involved in the binding at these two stages. Sperm binding to the zona was examined under a variety of conditions. Sperm were suspended in 0.9% NaCl or capacitated by two different methods. Eggs were immobilized on lectin-coated coverslips, compressed between a coverslip and a glass slide, or free in tissue culture dishes. The sperm-egg interaction was recorded on videotape or assessed after fixation of the eggs with bound sperm. Under all conditions studied, both acrosome-intact and acrosome-reacted sperm initiated binding to the zona. The binding was persistent and not transitory. In particular, acrosome-intact sperm that bound to the zona were observed to remain bound for up to 80 min. One acrosome-intact sperm, bound to the zona, was videotaped while it acrosome-reacted. When mixed sperm populations (on the average 24% acrosome-intact and 76% acrosome-reacted) were incubated with eggs for 30 min, an average of 10% of the bound sperm were acrosome-intact. The PH-20 monoclonal antibody has previously been shown to inhibit zona binding by guinea pig sperm of undetermined acrosomal status (P. Primakoff, H. Hyatt, and D. G. Myles (1985),J. Cell Biol.101,2239–2244). In this study, when the two populations of sperm were counted separately, PH-20 inhibited the binding of acrosome-reacted but not acrosome-intact sperm. Our results show that both acrosome-intact and acrosome-reacted guinea pig sperm can initiate binding to the zona; however, the binding in the two cases may not occur by the same mechanism.
岩藻多糖通过与内顶体膜和赤道区结合，抑制豚鼠精子附着在透明带上。
影响因子：
3.7
作者：
Huang,TT;Yanagimachi,R
通讯作者：
Yanagimachi,R
通过凝集素将哺乳动物卵固定在固体基质上，用于电子显微镜。
影响因子：
2
作者：
Cherr,GN;Cross,NL
通讯作者：
Cross,NL