Gastric mucosal ornithine decarboxylase: localization and stimulation by gastrin.
Gastric mucosal ornithine decarboxylase: localization and stimulation by gastrin.
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胃粘膜鸟氨酸脱羧酶：胃泌素的定位和刺激。
DOI：
10.1152/ajpgi.1988.255.3.g304
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Haddox,MK
中科院分区：
文献类型：
--
作者：
Johnson,LR;Tseng,CC;Tipnis,UR;Haddox,MK
注射胃泌素和再喂养禁食大鼠是胃泌酸腺粘膜的有效营养刺激。这两种刺激都没有增加组织中可检测到的鸟氨酸脱羧酶（ODC）活性。二氟甲基鸟氨酸（DFMO）是一种有效的ODC抑制剂，可阻断粘膜生长反应，表明ODC活性是生长所必需的。胃泌素给药后，在粘膜中可检测到亚精胺和精胺水平升高。使用高度特异性的，多克隆抗血清ODC，我们确定的酶是存在于泌酸腺粘膜局限于一个狭窄的带细胞的胃小凹和开口的腺体的基础。在胃窦粘膜中，ODC存在于整个粘膜的下部20%，其由颈部和幽门腺组成。使用抗血清稀释技术，我们表明，胃泌素管理增加免疫反应ODC在泌酸腺区，但不是在胃窦粘膜，在那里它没有营养作用。ODC的细胞含量升高在注射胃泌素后2小时内明显，在4小时达到峰值，并在12小时降至基础水平。胃泌素刺激的ODC增加仅限于窄带细胞，在对照动物中检测到低水平的酶蛋白。在泌酸腺粘膜提取物中检测到的脱羧活性不受DFMO或放线菌酮给药的抑制，DFMO或放线菌酮中的每一种都抑制其他组织中的ODC活性。向泌酸腺粘膜提取物的脱羧测定反应中添加未标记的赖氨酸可抑制放射性标记的鸟氨酸底物的脱羧。因此，胃很可能具有非特异性脱羧酶活性，这占了大部分测量的活性。（250字处删节）
Gastrin injection and refeeding fasted rats are effective trophic stimuli for the oxyntic gland mucosa of the stomach. Neither stimulus increases detectable ornithine decarboxylase (ODC) activity in the tissue. Difluoromethylornithine (DFMO), a potent inhibitor of ODC, blocks the mucosal growth response, indicating that ODC activity is necessary for growth. Elevated levels of spermidine and spermine are detectable in the mucosa after gastrin administration. Using a highly specific, polyclonal antiserum to ODC, we determined that the enzyme is present in oxyntic gland mucosa confined to a narrow band of cells at the base of the gastric pits and openings of the glands. In antral mucosa, ODC is present throughout the lower 20% of the mucosa, which consists of the necks and pyloric glands. Using antiserum dilution techniques, we show that gastrin administration increases immunoreactive ODC in the oxyntic gland area but not in the antral mucosa, where it has no trophic effect. Elevated cellular content of ODC is apparent within 2 h after injection of gastrin, peaks at 4 h, and declines to basal levels by 12 h. Gastrin-stimulated increase in ODC is confined to the narrow band of cells in which low levels of the enzyme protein were detected in control animals. The decarboxylating activity detectable in oxyntic gland mucosal extracts is not inhibited by administration of DFMO or cycloheximide, each of which inhibits ODC activity in other tissues. Addition of unlabeled lysine to the decarboxylation assay reaction of oxyntic gland mucosa extract inhibits the decarboxylation of radiolabeled ornithine substrate. Thus it is likely that the stomach possesses nonspecific decarboxylase activity, which accounts for most of the measured activity.(ABSTRACT TRUNCATED AT 250 WORDS)
肾功能不全、急性胰腺炎和大淀粉酶血症中淀粉酶的肾脏清除率。
影响因子：
39.2
作者：
M. Levitt;M. Rapoport;S. Cooperband
通讯作者：
S. Cooperband
泰国乳糖酶缺乏症：长期乳糖喂养的影响。
DOI：
10.1093/ajcn/22.5.638
发表时间：
1969
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
G. Keusch;F. Troncale;B. Thavaramara;P. Prinyanont;P. R. Anderson;N. Bhamarapravathi
通讯作者：
N. Bhamarapravathi
可溶性碳水化合物在大鼠和人结肠中的命运。
DOI：
10.1172/jci108383
发表时间：
1976
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
J. Bond;M. Levitt
通讯作者：
M. Levitt