Heterokaryon incompatibility is suppressed following conidial anastomosis tube fusion in a fungal plant pathogen.
Heterokaryon incompatibility is suppressed following conidial anastomosis tube fusion in a fungal plant pathogen.
复制标题
DOI：
10.1371/journal.pone.0031175
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Roca MG
中科院分区：
文献类型：
--
作者：
Ishikawa FH;Souza EA;Shoji JY;Connolly L;Freitag M;Read ND;Roca MG
在植物病原真菌和其他真菌中，菌丝融合后的水平基因/染色体转移和无性重组可能是导致植物病原真菌和其他真菌产生广泛遗传变异的原因。然而，营养(异核体)不亲和反应在阻止这些过程中的意义尚不清楚，这种反应通常会导致细胞死亡。在这项研究中，我们评估了在菌落起始和成熟菌落中不同类型的菌丝融合。我们使用营养亲和和不亲和的菜豆病原菌Colletotrichum lindemuthianum菌株，其中细胞核被绿色或红色荧光蛋白标记，以便在显微镜下监测菌丝融合后细胞核和异核细胞的命运。与在成熟菌落中菌丝融合会导致细胞在3h内死亡不同，在菌落启动过程中两个不相容菌株之间通过分生孢子吻合管(CAT)融合不会引起营养不亲和反应。相反，融合的分生孢子和胚芽存活下来，形成异核菌落，然后产生单核分生孢子，然后萌发形成具有不同于亲本菌株的表型特征的菌落。我们的结果表明，营养不亲和性反应在菌落启动过程中受到抑制。因此，CAT融合可能会使无性真菌增加其遗传多样性，并获得新的致病性状。
It has been hypothesized that horizontal gene/chromosome transfer and parasexual recombination following hyphal fusion between different strains may contribute to the emergence of wide genetic variability in plant pathogenic and other fungi. However, the significance of vegetative (heterokaryon) incompatibility responses, which commonly result in cell death, in preventing these processes is not known. In this study, we have assessed this issue following different types of hyphal fusion during colony initiation and in the mature colony. We used vegetatively compatible and incompatible strains of the common bean pathogen Colletotrichum lindemuthianum in which nuclei were labelled with either a green or red fluorescent protein in order to microscopically monitor the fates of nuclei and heterokaryotic cells following hyphal fusion. As opposed to fusion of hyphae in mature colonies that resulted in cell death within 3 h, fusions by conidial anastomosis tubes (CAT) between two incompatible strains during colony initiation did not induce the vegetative incompatibility response. Instead, fused conidia and germlings survived and formed heterokaryotic colonies that in turn produced uninucleate conidia that germinated to form colonies with phenotypic features different to those of either parental strain. Our results demonstrate that the vegetative incompatibility response is suppressed during colony initiation in C. lindemuthianum. Thus, CAT fusion may allow asexual fungi to increase their genetic diversity, and to acquire new pathogenic traits.
登录
查看更多内容
影响因子：
0.4
作者：
Barcelos, Q. L.;Souza, E. A.;Damasceno e Silva, K. J.
通讯作者：
Damasceno e Silva, K. J.
影响因子：
4.5
作者：
Coleman JJ;Rounsley SD;Rodriguez-Carres M;Kuo A;Wasmann CC;Grimwood J;Schmutz J;Taga M;White GJ;Zhou S;Schwartz DC;Freitag M;Ma LJ;Danchin EG;Henrissat B;Coutinho PM;Nelson DR;Straney D;Napoli CA;Barker BM;Gribskov M;Rep M;Kroken S;Molnár I;Rensing C;Kennell JC;Zamora J;Farman ML;Selker EU;Salamov A;Shapiro H;Pangilinan J;Lindquist E;Lamers C;Grigoriev IV;Geiser DM;Covert SF;Temporini E;Vanetten HD
通讯作者：
Vanetten HD
影响因子：
1.5
作者：
Clutterbuck, AJ
通讯作者：
Clutterbuck, AJ
影响因子：
1.5
作者：
Ishikawa, Francine H.;de Souza, Elaine A.;Davide, Livia M. C.
通讯作者：
Davide, Livia M. C.
DOI：
10.1590/s1413-70542010000100009
发表时间：
2010-02-01
期刊：
Ciência e Agrotecnologia
影响因子：
--
作者：
Ishikawa, Francine Hiromi;Barcelos, Quélen de Lima;Dias, Eustáquio Souza
通讯作者：
Dias, Eustáquio Souza