Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection.
Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection.
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DOI：
10.1002/anie.202107730
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发表时间：
2021-09-20
期刊：
影响因子：
--
通讯作者：
Pun SH
中科院分区：
文献类型：
--
作者：
Kacherovsky N;Yang LF;Dang HV;Cheng EL;Cardle II;Walls AC;McCallum M;Sellers DL;DiMaio F;Salipante SJ;Corti D;Veesler D;Pun SH
冠状病毒病2019年(新冠肺炎)大流行已经摧毁了全球的家庭，扰乱了医疗保健、经济和社会。针对不同病毒蛋白的分子识别试剂是快速诊断和靶向治疗的关键组件。在这项工作中，我们展示了新的DNA适配子的选择，它与SARS-CoV-2刺突糖蛋白结合具有高特异性和亲和力(~lt；80 nM)。通过结合实验和高分辨冷冻电子显微镜，我们证明了SNAP1(SARS-CoV-2刺突蛋白N-末端结构域结合适体1)与S的N-末端结构域结合。我们将SNAP1应用于侧向流动分析(LFA)和ELISA检测紫外线灭活的SARS-CoV-2病毒，检测浓度低至5×105拷贝/毫升。因此，SNAP1是一种很有前途的SARS-CoV-2诊断分子工具。快速诊断对于早期发现新冠肺炎和减少疾病传播至关重要。在本研究中，我们发现了DNA适配子SNAP1(SARS-CoV-2刺突蛋白N-末端结构域结合适配子1)，它与SARS-CoV-2刺突蛋白以及紫外线灭活的SARS-CoV-2病毒具有高亲和力和特异性。
The coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity (< 80 nM). Through binding assays and high resolution cryo-EM, we demonstrate that SNAP1 (SARS-CoV-2 spike protein N-terminal domain-binding aptamer 1) binds to the S N-terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV-inactivated SARS-CoV-2 at concentrations as low as 5 × 105 copies/mL. SNAP1 is therefore a promising molecular tool for SARS-CoV-2 diagnostics. Rapid diagnostics are essential for early detection of COVID-19 and reduced disease spread. In this study, we discovered the DNA aptamer SNAP1 (SARS-CoV-2 spike protein N-terminal domain binding aptamer 1), which binds with high affinity and specificity to SARS-CoV-2 spike protein as well as UV-inactivated SARS-CoV-2 virus.
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影响因子：
64.8
作者：
Ke, Zunlong;Oton, Joaquin;Briggs, John A. G.
通讯作者：
Briggs, John A. G.
DOI：
10.1126/science.aaz2193
发表时间：
2020-07-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chi Y;Remsik J;Kiseliovas V;Derderian C;Sener U;Alghader M;Saadeh F;Nikishina K;Bale T;Iacobuzio-Donahue C;Thomas T;Pe'er D;Mazutis L;Boire A
通讯作者：
Boire A
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
影响因子：
15
作者：
Kuai H;Zhao Z;Mo L;Liu H;Hu X;Fu T;Zhang X;Tan W
通讯作者：
Tan W
影响因子：
64.5
作者：
McCallum, Matthew;De Marco, Anna;Veesler, David
通讯作者：
Veesler, David