Granulocyte-macrophage colony stimulating factor suppresses lipopolysaccharide-induced osteoclast-like cell formation in mouse bone marrow cultures.
Granulocyte-macrophage colony stimulating factor suppresses lipopolysaccharide-induced osteoclast-like cell formation in mouse bone marrow cultures.
复制标题
粒细胞巨噬细胞集落刺激因子抑制小鼠骨髓培养物中脂多糖诱导的破骨细胞样细胞形成。
作者：
T. Shuto;E. Jimi;T. Kukita;M. Hirata;T. Koga
脂多糖（LPS）是一种有效的骨吸收因子。我们在三种类型的培养中研究了LPS对破骨细胞形成的影响。在不含地塞米松的全骨髓细胞培养中，LPS以剂量依赖的方式抑制1,25-二羟基维生素D3 [1,25(OH)2D3]诱导的破骨细胞形成。LPS增加了培养上清中粒细胞-巨噬细胞集落刺激因子（GM-CSF）的量，抗GM-CSF抗血清几乎消除了LPS对破骨细胞形成的抑制作用，提示LPS处理后产生的GM-CSF可能是抑制破骨细胞形成的原因。在地塞米松培养中，GM-CSF的量减少到单独使用125 (OH)2D3的三分之一，并且不受LPS处理的影响。在这个培养系统中，LPS增强了破骨细胞的形成。在含有125 (OH)2D3和地塞米松的非粘附骨髓细胞和基质细胞系共培养系统中，在上清中没有检测到GM-CSF的情况下，LPS显著增强破骨细胞的形成，而外源添加GM-CSF （100 pg/ml）几乎完全抑制破骨细胞的形成。LPS通过体外培养系统形成的破骨细胞样细胞刺激牙本质切片形成凹坑。
Lipopolysaccharide (LPS) is a potent bone resorbing factor. We investigated the effect of LPS on osteoclast formation in three types of cultures. LPS inhibited osteoclast formation induced by 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], in a dose-dependent manner, in cultures of whole bone marrow cells without dexamethasone. LPS increased the amount of granulocyte-macrophage colony stimulating factor (GM-CSF) in the culture supernatant, and anti-GM-CSF antiserum almost abolished the inhibition of osteoclast formation by LPS, thereby indicating that GM-CSF generated by treatment with LPS may be responsible for the inhibition of osteoclast formation. In cultures with dexamethasone, the amount of GM-CSF was decreased to one-third of that with 1,25(OH)2D3 alone and was not changed by treatment with LPS. In this culture system, LPS enhanced osteoclast formation. In the coculture system of nonadherent bone marrow cells and a stromal cell line in the presence of 1,25(OH)2D3 and dexamethasone, where no detectable GM-CSF was present in the supernatant, LPS markedly enhanced osteoclast formation, whereas exogenously added GM-CSF (100 pg/ml) almost completely inhibited osteoclast formation. LPS stimulated pit formation on dentin slices by the osteoclast-like cells formed by in vitro culture system.
影响因子：
56.9
作者：
SCHUMANN, RR;LEONG, SR;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
影响因子：
56.9
作者：
WRIGHT, SD;RAMOS, RA;MATHISON, JC
通讯作者：
MATHISON, JC