Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ.
Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ.
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单层上皮中细胞角蛋白的表达。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Herwig Ponstingl
中科院分区：
文献类型：
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作者：
Valentino Romano;M. Hatzfeld;T. Magin;R. Zimbelmann;W. W. Franke;Gernot Maier;Herwig Ponstingl
我们描述了编码人类细胞角蛋白的 mRNA 的 cDNA 克隆。 8和18，以及从它们的核苷酸序列推导出的氨基酸序列。人类细胞角蛋白编号8是基本（II型）亚家族的典型细胞角蛋白，与相应的牛和两栖动物（非洲爪蟾）蛋白高度同源；然而，与两栖动物蛋白不同，它的羧基末端“尾部”结构域不包含富含甘氨酸的寡肽重复序列。与广泛使用的血清诊断癌症标记物人类“组织多肽抗原”（TPA）的两个片段的氨基酸序列进行比较，揭示了序列同一性，表明该血清成分源自细胞内细胞角蛋白 1 号。 8 存在于多种上皮细胞和上皮源性肿瘤中。人类细胞角蛋白编号18 与相应的鼠蛋白非常相似，但在“头部”部分包含两个额外的 4 和 5 个氨基酸块。这些cDNA克隆和由其衍生的RNA探针用于通过来自各种癌和培养的癌细胞的RNA的Northern印迹测定来特异性检测mRNA。通过对含有肿瘤的组织（特别是含有转移性乳腺癌的淋巴结）的冷冻切片进行原位杂交，我们能够证明该过程的特异性和敏感性。讨论了能够原位检测编码给定细胞角蛋白多肽的 mRNA 对于细胞生物学研究和病理学的潜在价值。
We describe cDNA clones of mRNAs encoding human cytokeratins nos. 8 and 18, and the amino acid sequences deduced from their nucleotide sequences. Human cytokeratin no. 8 is a typical cytokeratin of the basic (type II) subfamily, which is highly homologous to the corresponding bovine and amphibian (Xenopus laevis) proteins; however, unlike the amphibian protein, it does not contain glycine-rich oligopeptide repeats in its carboxyterminal 'tail' domain. Comparison with the reported amino acid sequences of two fragments of human 'tissue polypeptide antigen' (TPA), a widely used serodiagnostic carcinoma marker, revealed sequence identity, indicating that this serum component is derived from the intracellular cytokeratin no. 8 present in diverse kinds of epithelia and epithelium-derived tumors. Human cytokeratin no. 18 is very similar to the corresponding murine protein but contains two additional blocks of 4 and 5 amino acids in the 'head' portion. These cDNA clones and the RNA probes derived therefrom were used to detect specifically mRNAs by Northern-blot assays of RNAs from various carcinomas and cultured carcinoma cells. Using in situ hybridization on frozen sections of tumor-containing tissues, notably lymph nodes containing metastatic breast carcinoma, we were able to demonstrate the specificity and sensitivity of this procedure. The potential value for cell-biological research and pathology of being able to detect a mRNA encoding a given cytokeratin polypeptide in situ is discussed.
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植入前小鼠胚胎中表达的 Endo B 细胞角蛋白的分子克隆和表征。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
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通讯作者：
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DOI：
10.1073/pnas.83.17.6377
发表时间：
1986
影响因子：
11.1
作者：
Robakis,NK;Sawh,PR;Wolfe,GC;Rubenstein,R;Carp,RI;Innis,MA
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Innis,MA
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DOI：
10.1073/pnas.82.14.4683
发表时间：
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影响因子：
11.1
作者：
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通讯作者：
Fuchs,E
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影响因子：
2.9
作者：
Ward,WS;Schmidt,WN;Schmidt,CA;Hnilica,LS
通讯作者：
Hnilica,LS
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影响因子：
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作者：
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通讯作者：
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