Genetic analyses for dermatitis and IgE hyperproduction in the NC/Nga mouse
Genetic analyses for dermatitis and IgE hyperproduction in the NC/Nga mouse
复制标题
NC/Nga 小鼠皮炎和 IgE 过度产生的遗传分析
DOI：
10.1007/s002510050330
复制
发表时间：
1997
期刊：
影响因子：
3.2
通讯作者：
H. Matsuda
中科院分区：
文献类型：
--
作者：
Masaoki Tsudzuki;N. Watanabe;A. Wada;Y. Nakane;J. Hiroi;H. Matsuda
Atopic dermatitis (AD) is a severe problem in the human species, and many approaches have been taken to reveal the causes of the disease and to develop therapeutical methods to combat it. However, the fundamental causes of AD are still unknown, and no radical therapy exists (Leung 1997; van Bever 1992), mainly due to lack of a suitable animal model for studying the mechanisms through which AD arises. We recently determined that the NC/Nga (NC) mice would be an excellent animal model for human AD, because they show severe hereditary dermatitis with hyperproduction of immunoglobulin E (IgE), similar to the situation in humans (Matsuda et al. 1997). The NC strain was established as an inbred strain by K. Kondo in 1957 based on Japanese fancy mice (Festing 1996; Kondo 1984), and is now a highly inbred strain (more than 100 generations). The dermatitis and IgE hyperproduction occur spontaneously in all NC mice reared under non-sterile (conventional) conditions, but no genetic analyses for these characters have been done so far. When trying to use the NC mice as an efficient animal model for human AD, it is necessary to reveal the mode of inheritance of the expression of dermatitis and IgE hyperproduction, and we describe it in this paper. All animals used in this study were handled according to the rules described in Guide for the Care and Use of Laboratory Animals (NIH publication no. 86-23, 1985); Standards Relating to the Care and Management of Experimental Animals, Prime Minister's Office, Japan, 1980; and Guide for the Use of Experimental Animals in Universities, The Ministry of Education, Science, and Culture, Japan, 1987. NC and BALB/cA (BALB) strains and their crossbreds were used, with the BALB mice serving as a control. All mice were raised in a clean, conventional animal room with temperature and relative humidity adjusted to 23+2 °C and 50+10%, respectively. The room was illuminated by fluorescent light from 5 am until 9 pm. Mice were kept in a polycarbonate cage (Clea Japan, Inc., Osaka, Japan) that was bedded with clean wood chips (Clea Japan, Inc.), and tap water and a commercial diet for small rodents (CE-2, Clea Japan, Inc.) were provided for ad libitum consumption. We reciprocally paired NC mice with BALB mice to produce F1 progeny. Subsequently, F2 and backcross generations were also produced. In these mice, we scored the incidence of abnormal mice showing dermatitis or high levels of plasmic total IgE at 12 weeks of age. The resulting segregation ratios were analyzed by the chi-square test. To obtain segregation data for the mice with dermatitis, we classified the animals on the basis of the absence or presence of scratching, erythema, hemorrhage, edema, superficial erosion, deep excoriation, and/or scaling and dryness of the skin, because all conventional NC mice are characterized by these symptoms. The BALB mice, however, showed no such symptoms even though they were reared together with the severely lesioned NC mice in the same cage for several months (Matsuda et al. 1997). To obtain segregation data for the mice showing IgE hyperproduction, we classified the animals with an IgE level less than 1500 ng/ml as a low-level group and those with more than 9000 ng/ml as a high-level group, because the F2 and M. Tsudzuki ( ) Laboratory of Animal Breeding and Genetics, Faculty of Applied Biological Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739, Japan
影响因子：
56.9
作者：
MARSH, DG;NEELY, JD;BEATY, TH
通讯作者：
BEATY, TH
使用淋巴器官的细胞提取物通过被动皮肤过敏反应检测 IgE 抗体形成细胞。
影响因子：
2.2
作者：
Watanabe,N;Kobayashi,A;Miyajima,H;Hirano,T;Ovary,Z
通讯作者：
Ovary,Z
特应性皮炎：免疫生物学和免疫调节剂治疗。
DOI：
--
发表时间：
1997
期刊：
Clinical and experimental immunology.
影响因子：
--
作者：
Leung,DY
通讯作者：
Leung,DY