Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea.
Diversity and abundance of ammonia-oxidizing prokaryotes in sediments from the coastal Pearl River estuary to the South China Sea.
复制标题
珠江口沿岸至南海沉积物中氨氧化原核生物的多样性和丰度
DOI：
10.1007/s10482-011-9610-1
复制
发表时间：
2011-11
影响因子：
2.6
通讯作者：
Gu, Ji-Dong
中科院分区：
文献类型：
--
作者：
Cao, Huiluo;Hong, Yiguo;Li, Meng;Gu, Ji-Dong
关键词：
以珠江口沿岸至南海深水陆坡为断面，研究了硝化微生物（包括氨氧化古菌（AOA）和β-变形菌（beta-AOB））的多样性和丰度，并对影响硝化微生物的理化参数进行了沿着分析。通过检测氨单加氧酶亚基A（theamoA）基因，确定硝化微生物多样性。AOA和beta-AOB的群落结构从近岸边缘区向深海斜坡区发生了明显的转移，AOAamoA的OTU数比beta-AOB的OTU数更稳定。在深海中，β-氨氧化细菌的OTU值随距离的增加而增加。污染区β-氨氧化物的多样性较低，只有优势菌株，而清洁区β-氨氧化物的多样性较高，没有优势菌株。此外，β-AOB的多样性与pH值相关，而AOA与理化参数之间没有明显的关系。β-氨氧化物对样带环境变化更为敏感，可能是环境变化的潜在指示物。此外，南海表层沉积物中的AOA和beta-AOB类型多样且独特，与其他环境不同，表明南海存在硝化原核生物。
In the present study the diversity and abundance of nitrifying microbes including ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) were investigated, along with the physicochemical parameters potentially affecting them, in a transect of surface sediments from the coastal margin adjacent to the Pearl River estuary to the slope in the deep South China Sea. Nitrifying microbial diversity was determined by detecting theamoA(ammonia monooxygenase subunit A) gene. An obvious community structure shift for both AOA and beta-AOB from the coastal marginal areas to the slope in the deep-sea was detected, while the OTU numbers of AOAamoAwere more stable than those of the beta-AOB. The OTUs of beta-AOB increased with the distance from the coastal margin areas to the slope in the deep-sea. Beta-AOB showed lower diversity with dominant strains in a polluted area but higher diversity without dominant strains in a clean area. Moreover, the diversity of beta-AOB was correlated with pH values, while no noticeable relationships were established between AOA and physicochemical parameters. Beta-AOB was more sensitive to transect environmental variability and might be a potential indicator for environmental changes. Additionally, the surface sediments surveyed in the South China Sea harboured diverse and distinct AOA and beta-AOB phylotypes different from other environments, suggesting the endemicity of some nitrifying prokaryotes in the South China Sea.
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影响因子：
5.1
作者：
Mosier, Annika C.;Francis, Christopher A.
通讯作者：
Francis, Christopher A.
影响因子：
4.4
作者：
Beman, J. Michael;Francis, Christopher A.
通讯作者：
Francis, Christopher A.
红树林沉积物中氨氧化古菌（AOA）和氨氧化细菌（AOB）的空间分布和丰度
影响因子：
5
作者：
Li, Meng;Cao, Huiluo;Hong, Yiguo;Gu, Ji-Dong
通讯作者：
Gu, Ji-Dong
长江口及东海沉积物氨氧化缝隙菌多样性及空间分布对河口和环境梯度的响应
影响因子：
2.8
作者：
Dang, Hongyue;Zhang, Xiaoxia;Yang, Guanpin
通讯作者：
Yang, Guanpin
影响因子：
3
作者：
Lozupone C;Hamady M;Knight R
通讯作者：
Knight R