Regulation of bacterial priming and daughter strand synthesis through helicase-primase interactions.
Regulation of bacterial priming and daughter strand synthesis through helicase-primase interactions.
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DOI：
10.1093/nar/gkl363
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发表时间：
2006
影响因子：
14.9
通讯作者：
Berger, James M.
中科院分区：
文献类型：
--
作者：
Corn, Jacob E.;Berger, James M.
复制体是一种多组件分子机器，负责快速准确地复制生物体的基因组。细菌复制体的核心成员是 DnaB（复制解旋酶），它分离亲本双链体，为新合成的子链提供模板。一种独特的 RNA 聚合酶 DnaG 引物酶与 DnaB 结合，在每个复制周期的滞后链上重复启动数千个冈崎片段。多项研究表明，DnaG 和 DnaB 相互作用的稳定性和频率决定了冈崎片段的长度。最近的研究表明，每个 DnaB 六聚体与多个 DnaG 分子结合，并且这些引物酶可以相互协调以调节其在复制叉处的活性。总之，不同的证据开始表明冈崎片段的起始可能部分受到与解旋酶结合的多个引物酶之间的串扰的控制。
The replisome is a multi-component molecular machine responsible for rapidly and accurately copying the genome of an organism. A central member of the bacterial replisome is DnaB, the replicative helicase, which separates the parental duplex to provide templates for newly synthesized daughter strands. A unique RNA polymerase, the DnaG primase, associates with DnaB to repeatedly initiate thousands of Okazaki fragments per replication cycle on the lagging strand. A number of studies have shown that the stability and frequency of the interaction between DnaG and DnaB determines Okazaki fragment length. More recent work indicates that each DnaB hexamer associates with multiple DnaG molecules and that these primases can coordinate with one another to regulate their activities at a replication fork. Together, disparate lines of evidence are beginning to suggest that Okazaki fragment initiation may be controlled in part by crosstalk between multiple primases bound to the helicase.
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影响因子：
2.9
作者：
Biswas, EE;Biswas, SB
通讯作者：
Biswas, SB
影响因子：
3.2
作者：
GROMPE, M;VERSALOVIC, J;LUPSKI, JR
通讯作者：
LUPSKI, JR
影响因子：
16
作者：
Kato, M;Ito, T;Ellenberger, T
通讯作者：
Ellenberger, T
影响因子：
64.8
作者：
Lee, JB;Hite, RK;van Oijen, AM
通讯作者：
van Oijen, AM
DOI：
10.1073/pnas.59.2.598
发表时间：
1968-01-01
影响因子：
11.1
作者：
OKAZAKI, R;OKAZAKI, T;SUGINO, A
通讯作者：
SUGINO, A