Base‐specific separation of oligodeoxynucleotides by capillary affinity gel electrophoresis
Base‐specific separation of oligodeoxynucleotides by capillary affinity gel electrophoresis
复制标题
通过毛细管亲和凝胶电泳对寡脱氧核苷酸进行碱基特异性分离
作者：
Y. Baba;T. Sawa;A. Kishida;M. Akashi
毛细管亲和凝胶电泳应用于寡脱氧核苷酸的序列特异性和碱基组成特异性识别，利用固定在毛细管凝胶中的核酸类似物与具有不同序列的可溶性寡脱氧核苷酸之间形成异源双链体。使用填充有聚丙烯酰胺和合成核酸类似物[聚（9-乙烯基腺嘌呤）]的缀合凝胶的毛细管进行毛细管亲和凝胶电泳，可有效地从A6、C6、G6和T6的四种均聚物的混合物中选择性分离六胸苷酸，并完全分离六脱氧核苷酸的五种杂聚物（TAAAAA、TTAAAA、TTTAAA、TTTTAA、TTTTTA）。我们还证明了毛细管亲和凝胶电泳可用于DNA序列异构体（TTTTAA、TTTTAAT、TTTATA和TTTTAA）的选择性和灵敏的序列特异性识别。
Capillary affinity gel electrophoresis was applied to sequence‐specific and base composition‐specific recognition of oligodeoxynucleotides, utilizing the formation of heteroduplexes between a nucleic acid analogue immobilized into the capillary gel and soluble oligodeoxynucleotides with different sequences. Capillary affinity gel electrophoresis using capillaries filled with a conjugated gel of polyacrylamide and a synthetic nucleic acid analogue [poly(9‐vinyladenine)] was effective for the selective separation of hexathymidylic acid from a mixture of four homopolymers of A6, C6, G6, and T6 and for the complete resolution of five heteropolymers of hexadeoxynucleotides (TAAAAA, TTAAAA, TTTAAA, TTTTAA, TTTTTA). We also demonstrated that capillary affinity gel electrophoresis was useful for the selective and the sensitive sequence‐specific recognition of sequence isomers of DNA (TTTTAA, TTTTAAT, TTTATA, and TTTTAA).
DOI：
10.1016/s0021-9673(00)91270-6
发表时间：
1990-06-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
GOSS, TA;BARD, M;JARRETT, HW
通讯作者：
JARRETT, HW