The indole pulse: a new perspective on indole signalling in Escherichia coli.
The indole pulse: a new perspective on indole signalling in Escherichia coli.
复制标题
DOI：
10.1371/journal.pone.0093168
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Summers DK
中科院分区：
文献类型：
--
作者：
Gaimster H;Cama J;Hernández-Ainsa S;Keyser UF;Summers DK
吲哚具有多种信号作用，包括调节生物膜形成、毒力和应激反应。0.5-1.0 mM的吲哚浓度可引起变化，类似于大肠杆菌固定相培养的上清。在这里，我们描述了另一种吲哚信号传导模式，促进大肠杆菌细胞在长期静止期的存活。失去产生吲哚能力的突变体在这些条件下的存活率降低。值得注意的是，在培养上清液中添加1mm吲哚不足以恢复突变体的长期存活。我们提供的证据表明，在这种情况下，相关信号不是培养上清中的1mm吲哚，而是从指数生长过渡到固定阶段的细胞内吲哚的瞬态脉冲。在此脉冲期间，细胞相关的吲哚达到最大约60毫米。我们认为，这足以通过基于离子载体的机制抑制生长和分裂，并导致细胞在资源耗尽之前进入静止期。在长时间的饥饿中，未使用的资源被用来修复和维持细胞。
Indole has diverse signalling roles, including modulation of biofilm formation, virulence and stress responses. Changes are induced by indole concentrations of 0.5–1.0 mM, similar to those found in the supernatant of Escherichia coli stationary phase culture. Here we describe an alternative mode of indole signalling that promotes the survival of E. coli cells during long-term stationary phase. A mutant that has lost the ability to produce indole demonstrates reduced survival under these conditions. Significantly, the addition of 1 mM indole to the culture supernatant is insufficient to restore long-term survival to the mutant. We provide evidence that the pertinent signal in this case is not 1 mM indole in the culture supernatant but a transient pulse of intra-cellular indole at the transition from exponential growth to stationary phase. During this pulse the cell-associated indole reaches a maximum of approximately 60 mM. We argue that this is sufficient to inhibit growth and division by an ionophore-based mechanism and causes the cells to enter stationary phase before resources are exhausted. The unused resources are used to repair and maintain cells during the extended period of starvation.
登录
查看更多内容
影响因子：
2.9
作者：
Kamaraju, Kishore;Smith, Jacqueline;Wang, Jingxin;Roy, Varnika;Sintim, Herman O.;Bentley, William E.;Sukharev, Sergei
通讯作者：
Sukharev, Sergei
影响因子：
3.6
作者：
Hirakawa, H;Inazumi, Y;Yamaguchi, A
通讯作者：
Yamaguchi, A
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
DOI：
10.1073/pnas.48.8.1431
发表时间：
1962-01-01
影响因子：
11.1
作者：
NEWTON, WA;SNELL, EE
通讯作者：
SNELL, EE
影响因子：
3.6
作者：
PATIENT, ME;SUMMERS, DK
通讯作者：
SUMMERS, DK