Aleutian mink disease virus in furbearing mammals in Nova Scotia, Canada.
Aleutian mink disease virus in furbearing mammals in Nova Scotia, Canada.
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DOI：
10.1186/1751-0147-55-10
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发表时间：
2013-02-08
影响因子：
2.1
通讯作者：
Farid AH
中科院分区：
文献类型：
--
作者：
Farid AH
阿留申水貂病病毒（AMDV）在加拿大的牧场和自由放养的美国水貂中广泛传播，但没有关于其在其他野生动物物种中流行的信息。本文描述了加拿大新斯科舍省12种毛皮动物的AMDV流行情况。从2009年11月至2011年2月期间在10个NS县捕获的12种毛皮动物的462只野生动物尸体中收集样品。使用两对引物通过PCR检测病毒DNA，并通过脾匀浆上的对流免疫电泳（CIEP）检测抗病毒抗体。在60只美国水貂中有56只（93.3%）、61只短尾鼬鼠中有43只（70.5%）、8只条纹臭鼬中有2只（25.0%）、11只北美水獭中有2只（18.2%）、85只浣熊中有9只（10.6%）、20只山猫中有2只（10.0%）检测到PCR或CIEP阳性。来自6名渔民、24只郊狼、25只红狐、58只海狸、45只红松鼠和59只麝鼠的样本呈阴性。CIEP检测到56只水貂中的16只（28.6%）和8只臭鼬中的1只（12.5%）存在AMDV抗体。13只水貂的PCR和CIEP均为阳性，3只水貂和1只臭鼬的CIEP为阳性，而PCR为阴性。阳性CIEP或PCR动物存在于所有9个县，貂或黄鼠狼样本收集。AMDV在全省如此多的物种中的存在具有重要的流行病学后果，并可能对圈养水貂以及易感野生动物造成严重的健康问题。野生动物和圈养水貂之间的病毒传播机制以及AMDV暴露对易感物种生存能力的影响值得进一步研究。
Aleutian mink disease virus (AMDV) is widespread among ranched and free-ranging American mink in Canada, but there is no information on its prevalence in other wild animal species. This paper describes the prevalence of AMDV of 12 furbearing species in Nova Scotia (NS), Canada. Samples were collected from carcasses of 462 wild animals of 12 furbearing species, trapped in 10 NS counties between November 2009 and February 2011. Viral DNA was tested by PCR using two primer pairs, and anti-viral antibodies were tested by counterimmunoelectrophoresis (CIEP) on spleen homogenates. Positive PCR or CIEP samples were detected in 56 of 60 (93.3%) American mink, 43 of 61 (70.5%) short-tailed weasels, 2 of 8 (25.0%) striped skunks, 2 of 11 (18.2%) North American river otters, 9 of 85 (10.6%) raccoons, and 2 of 20 (10.0%) bobcats. Samples from six fishers, 24 coyotes, 25 red foxes, 58 beavers, 45 red-squirrels and 59 muskrats were negative. Antibodies to AMDV were detected by CIEP in 16 of 56 (28.6%) mink and one of the 8 skunks (12.5%). Thirteen of the mink were positive for PCR and CIEP, but three mink and one skunk were CIEP positive and PCR negative. Positive CIEP or PCR animals were present in all nine counties from which mink or weasel samples were collected. The presence of AMDV in so many species across the province has important epidemiological ramifications and could pose a serious health problem for the captive mink, as well as for susceptible wildlife. The mechanism of virus transmission between wildlife and captive mink and the effects of AMDV exposure on the viability of the susceptible species deserve further investigation.
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影响因子：
14.9
作者：
Aljanabi, SM;Martinez, I
通讯作者：
Martinez, I
影响因子：
5.4
作者：
BLOOM, ME;RACE, RE;WOLFINBARGER, JB
通讯作者：
WOLFINBARGER, JB
影响因子：
2.4
作者：
Pennick, K. E.;Latimer, K. S.;Sarver, C. F.
通讯作者：
Sarver, C. F.
影响因子：
1.3
作者：
Mañas, S;Ceña, JC;Bloom, ME
通讯作者：
Bloom, ME
影响因子：
3.1
作者：
ALEXANDERSEN, S;HAU, J
通讯作者：
HAU, J