Altered choroid plexus gene expression in major depressive disorder.
Altered choroid plexus gene expression in major depressive disorder.
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DOI：
10.3389/fnhum.2014.00238
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发表时间：
2014
影响因子：
2.9
通讯作者：
Watson SJ
中科院分区：
文献类型：
--
作者：
Turner CA;Thompson RC;Bunney WE;Schatzberg AF;Barchas JD;Myers RM;Akil H;Watson SJ
鉴于对情绪障碍生物标志物的迫切兴趣，我们评估了重度抑郁症（MDD）患者脉络丛（CP）中的基因表达，CP是产生脑脊液（CSF）的区域。在CP中表达的基因可以分泌到CSF中，并且可能是潜在的生物标志物候选物。鉴于我们之前已经表明成纤维细胞生长因子家族成员在MDD受试者死后的大脑中差异表达，并且CP是大脑中生长因子的已知来源，我们提出了在MDD受试者的CP中是否会发现生长因子失调的问题。我们进行了激光捕获显微镜与MDD和精神正常对照组在海马水平的CP。然后，我们提取、扩增、标记cRNA并将其与Illumina BeadChips杂交以评估基因表达。在对照组中，已知最丰富的转录本是甲状腺素运载蛋白。此外，对照组中14个最高表达的转录本中有一半编码核糖体蛋白。使用BeadStudio软件，我们鉴定了对照和MDD样品之间差异表达的169个转录物（p < 0.05）。使用通路分析，我们注意到MDD受试者中改变的顶级网络包括转化生长因子β（TGFβ）通路的多个成员。定量实时PCR（qRT-PCR）证实了MDD受试者中与细胞外基质相互作用的几种转录物的下调。这些结果表明，MDD受试者的CP中可能存在细胞骨架改变，可能导致血-CSF-脑屏障破坏。
Given the emergent interest in biomarkers for mood disorders, we assessed gene expression in the choroid plexus (CP), the region that produces cerebrospinal fluid (CSF), in individuals with major depressive disorder (MDD). Genes that are expressed in the CP can be secreted into the CSF and may be potential biomarker candidates. Given that we have previously shown that fibroblast growth factor family members are differentially expressed in post-mortem brain of subjects with MDD and the CP is a known source of growth factors in the brain, we posed the question whether growth factor dysregulation would be found in the CP of subjects with MDD. We performed laser capture microscopy of the CP at the level of the hippocampus in subjects with MDD and psychiatrically normal controls. We then extracted, amplified, labeled, and hybridized the cRNA to Illumina BeadChips to assess gene expression. In controls, the most highly abundant known transcript was transthyretin. Moreover, half of the 14 most highly expressed transcripts in controls encode ribosomal proteins. Using BeadStudio software, we identified 169 transcripts differentially expressed (p < 0.05) between control and MDD samples. Using pathway analysis we noted that the top network altered in subjects with MDD included multiple members of the transforming growth factor-beta (TGFβ) pathway. Quantitative real-time PCR (qRT-PCR) confirmed downregulation of several transcripts that interact with the extracellular matrix in subjects with MDD. These results suggest that there may be an altered cytoskeleton in the CP in MDD subjects that may lead to a disrupted blood-CSF-brain barrier.
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DOI：
10.1073/pnas.0904635106
发表时间：
2009-07-14
影响因子：
11.1
作者：
Grimm, P. Richard;Irsik, Debra L.;Sansom, Steven. C.
通讯作者：
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DOI：
10.1016/j.pnpbp.2013.03.005
发表时间：
2013-07-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
3.5
作者：
Li, JZ;Vawter, MP;Myers, RM
通讯作者：
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影响因子：
3
作者：
JONES, EG;HENDRY, SHC;TOURTELLOTTE, WW
通讯作者：
TOURTELLOTTE, WW