Fabrication of Nanoindented Electrodes for Glucose Detection
Fabrication of Nanoindented Electrodes for Glucose Detection
复制标题
用于葡萄糖检测的纳米压痕电极的制造
DOI：
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发表时间：
2010
影响因子：
5
通讯作者：
G. Slaughter
中科院分区：
文献类型：
--
作者：
G. Slaughter
背景资料：本文的目的是设计，制造和评估一种新型的葡萄糖生物传感器的基础上使用原子力显微镜创建纳米压痕电极（NIDES）的选择性检测葡萄糖。研究方法：原子力显微镜纳米压痕技术扩展到共价固定葡萄糖氧化酶NIDE通过复合水凝胶膜组成的互穿网络的固有导电聚（3，4-乙撑二氧噻吩）四甲基丙烯酸酯内生长的紫外线交联的羟乙基甲基丙烯酸酯为基础的水凝胶在体外安培NIDE生物传感器的长期监测葡萄糖。结果如下：葡萄糖的线性范围为0.25 ~ 20 mM，灵敏度为0.32 μA/mM，响应时间小于5 s。存在的竞争性异构体（果糖）没有干扰;唯一的干扰来自与H2 O2（抗坏血酸）反应的物质。线性方程为：B响应（μA）= 0.323 [葡萄糖]（mM）+ 0.634（μA），n = 24，r2 = 0.994。结论：结果表明，所制备的NIDE葡萄糖生物传感器的动态范围、灵敏度和响应时间均有所提高，对葡萄糖具有良好的检测性能。
Background: The objective of this article was to design, fabricate, and evaluate a novel type of glucose biosensors based on the use of atomic force microscopy to create nanoindented electrodes (NIDEs) for the selective detection of glucose. Methods: Atomic force microscopy nanoindentation techniques were extended to covalently immobilized glucose oxidase on NIDEs via composite hydrogel membranes composed of interpenetrating networks of inherently conductive poly(3,4-ethylenedioxythiophene) tetramethacrylate grown within ultraviolet cross-linked hydroxyethylmethacrylate-based hydrogels to produce an in vitro amperometric NIDE biosensor for the long-term monitoring of glucose. Results: The calibration curve for glucose was linear from 0.25 to 20 mM. Results showed that the NIDE glucose biosensor has a much higher detection sensitivity of 0.32 μA/mM and rapid response times (<5 seconds). There was no interference from the competing interferent (fructose) present; the only interference was from species that react with H2O2 (ascorbic acid). The linear equation was B response (μA) = 0.323 [glucose] (mM) + 0.634 (μA); n = 24, r 2 = 0.994. Conclusion: Results showed that the resultant NIDE glucose biosensor increases the dynamic range, device sensitivity, and response time and has excellent detecting performance for glucose.
影响因子：
10.8
作者：
Peppas, NA;Keys, KB;Lowman, AM
通讯作者：
Lowman, AM