Proviral genome of radiation leukemia virus: molecular cloning of biologically active proviral DNA and nucleotide sequence of its long terminal repeat
Proviral genome of radiation leukemia virus: molecular cloning of biologically active proviral DNA and nucleotide sequence of its long terminal repeat
复制标题
放射性白血病病毒原病毒基因组：生物活性原病毒DNA及其长末端重复核苷酸序列的分子克隆
作者：
M. Janowski;J. Merregaert;J. Boniver;J. Maisin
将一种致白血病性和促胸腺性C57 BL/Ka小鼠逆转录病毒RadLV/VL 3（T+L+）的前病毒基因组克隆为细菌质粒pBR 322中的生物活性Pst I插入片段。它的限制性酶切图谱与已知的相同小鼠品系的两种非嗜胸腺性和非致白血病性病毒的图谱进行了比较：亲嗜性BL/Ka（B）病毒和放射性白血病病毒复合物的异嗜性组分。在gag-pol基因连接处周围、pol基因和env基因中观察到差异。此外，RadLV/VL 3（T+L+）长末端重复序列的核苷酸序列揭示存在两个拷贝的43个碱基对序列，其中BL/Ka（B）只有一个拷贝。
The proviral genome of a leukemogenic and thymotropic C57BL/Ka mouse retrovirus, RadLV/VL3(T+L+), was cloned as a biologically active PstI insert in the bacterial plasmid pBR322. Its restriction map was compared with those, already known, of two nonthymotropic and nonleukemogenic viruses of the same mouse strain: the ecotropic BL/Ka(B) virus and the xenotropic constituent of the radiation leukemia virus complex. Differences were observed around the gag-pol gene junction, in the pol gene, and in the env gene. Moreover, the nucleotide sequence of the RadLV/VL3(T+L+) long terminal repeat revealed the existence of two copies of a 43-base-pair sequence, of which BL/Ka(B) possesses only one copy.
C57BL/ka 小鼠放射性白血病病毒 (RadLV) 和相关逆转录病毒的分子研究。
DOI：
10.1016/s0079-6603(08)60431-6
发表时间：
1983
期刊：
Progress in nucleic acid research and molecular biology
影响因子：
--
作者：
Grymes,RA;Scott,ML;Kim,JP;Fry,KE;Kaplan,HS
通讯作者：
Kaplan,HS
Friend 脾病灶形成病毒膜糖蛋白结构基因突变导致致白血病性丧失。
DOI：
10.1073/pnas.80.15.4704
发表时间：
1983
影响因子：
11.1
作者：
Ruta,M;Bestwick,R;Machida,C;Kabat,D
通讯作者：
Kabat,D