AII amacrine cells limit scotopic acuity in central macaque retina: A confocal analysis of calretinin labeling
AII amacrine cells limit scotopic acuity in central macaque retina: A confocal analysis of calretinin labeling
复制标题
所有无长突细胞限制中央猕猴视网膜的暗视敏锐度：钙视网膜蛋白标记的共聚焦分析
DOI：
--
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Massey
中科院分区：
文献类型：
--
作者：
S. Mills;S. Massey
我们使用calretinin抗体选择性地标记猕猴视网膜中AII无毛细胞的嵌合。双标记材料的共聚焦分析表明，AII树突在包围突触末梢之前，在下降杆双极轴突周围螺旋下降。来自先前观察到的内核层多巴胺能丛的过程被观察到与calretinin阳性的AII体细胞接触。细胞内神经生物素注射显示，AII无突细胞与其他AII无突细胞和一些未识别的锥体双极细胞有示踪偶联。对猕猴AII无突细胞的视网膜分布（包括中央凹内和周围的区域）的分析显示，在偏心距为1.5 mm时，峰值密度约为5,000个细胞/mm2。用共聚焦显微镜证实了calretinin抗体对中央视网膜AII无毛细胞的染色。这些结果表明，calretinin抗体可用于选择性地标记AII amacrine细胞群，并且灵长类AII amacrine细胞具有许多先前描述的哺乳动物AII amacrine细胞的特征。AII细胞的峰值密度与暗视灵敏度的峰值采样率基本一致。计算表明，在猕猴视网膜中央，小神经节细胞数量较多，所有的无突细胞形成暗视的极限（Wässle et al. [1995] J. Comp. Neurol. 361:537-551）。当神经节细胞密度从中央凹迅速下降时，在偏心率15°左右有一个交叉点，这与心理物理学得出的暗位视力与偏心率图的拐点相匹配（Lennie and Fairchild [1994] Vision re . 34:477-482）。解剖和心理物理数据之间的对应关系支持我们的解释，即AII无突细胞的解剖采样率限制了中央暗位敏锐度。[j]中华神经科杂志，1999。©1999 Wiley‐Liss, Inc。
We have used calretinin antibodies to label selectively the mosaic of AII amacrine cells in the macaque retina. Confocal analysis of double‐labeled material indicated that AII dendrites spiral down around descending rod bipolar axons before enveloping the synaptic terminals. Processes from a previously observed dopaminergic plexus in the inner nuclear layer were observed to contact the somata of calretinin‐positive AII somata. Intracellular neurobiotin injection revealed that AII amacrine cells are tracer coupled to other AII amacrine cells and to some unidentified cone bipolar cells. An analysis of the retinal distribution of macaque AII amacrine cells, including an area in and around the fovea, showed a peak density of approximately 5,000 cells/mm2 at an eccentricity of 1.5 mm. Staining of AII amacrine cells in central retina with antibodies to calretinin was confirmed by confocal microscopy. These results indicate that calretinin antibodies can be used to label the AII amacrine cell population selectively and that primate AII amacrine cells share many of the features of previously described mammalian AII amacrine cells. The peak AII cell density closely matched the peak sampling rate of scotopic visual acuity. Calculations suggest that, in central macaque retina, where midget ganglion cells are more numerous, AII amacrine cells form the limit of scotopic visual acuity (Wässle et al. [1995] J. Comp. Neurol. 361:537–551). As the ganglion cell density falls rapidly away from the fovea, there is a cross‐over point at around 15° eccentricity that matches the inflection point in a psychophysically derived plot of scotopic visual acuity versus eccentricity (Lennie and Fairchild [1994] Vision Res. 34:477–482). The correspondence between the anatomic and psychophysical data supports our interpretation that the anatomic sampling rate of AII amacrine cells limits central scotopic acuity. J. Comp. Neurol. 411:19–34, 1999. © 1999 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.93.25.14906
发表时间：
1996-12
影响因子：
11.1
作者：
E. Strettoi;R. Masland
通讯作者：
E. Strettoi;R. Masland