Characterization of two mannose-binding protein cDNAs from rhesus monkey (Macaca mulatta): structure and evolutionary implications.
Characterization of two mannose-binding protein cDNAs from rhesus monkey (Macaca mulatta): structure and evolutionary implications.
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恒河猴 (Macaca mulatta) 的两种甘露糖结合蛋白 cDNA 的表征：结构和进化意义。
DOI：
10.1093/glycob/6.5.543
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发表时间：
1996
期刊：
影响因子：
4.3
通讯作者：
Sastry,KN
中科院分区：
文献类型：
--
作者：
Mogues,T;Ota,T;Tauber,AI;Sastry,KN
甘露糖结合蛋白（MBPs）是集合家族的成员，被认为是多种病毒和细菌的凝集素调理素。两种截然不同但相关的MBPs， MBP-A和MBP-C，在氨基酸水平上具有-55%的同源性，先前已经从啮齿动物中鉴定出来。然而，在人类中，只有一种形式的MBP已被表征。在本文中，我们报道了阐明灵长类动物MBPs进化的研究。ELISA和Western blot分析表明，恒河猴和食蟹猴的血清中有两种形式的MBP，而黑猩猩只有一种形式，与人类相似。从恒河猴肝脏cDNA文库中分离出两个不同的MBP cDNA克隆，并对其进行了鉴定。恒河猴的MBP-A与小鼠和大鼠的MBP-A密切相关，在氨基酸水平上分别具有77%和75%的同源性。恒河猴的MBP- a在n端也有3个半胱氨酸，类似于小鼠和大鼠的MBP- a和人的MBP。恒河鼠MBP- c在氨基酸水平上与人类MBP有90%的相似性，在n端有三个半胱氨酸，而啮齿动物的MBP- c只有两个半胱氨酸残基。在小鼠和大鼠的MBP-A中都不存在，但在啮齿动物的MBP-C、鸡和人的MBPs中存在，在恒河猴的MBP-A中也发现了靠近n端的9个氨基酸。恒河猴和其他哺乳动物MBPs的系统发育分析，结合血清学数据表明，至少有两个不同的MBP基因在哺乳动物辐射之前存在，而类人猿祖先显然失去了其中一个基因或无法表达。
Mannose-binding proteins (MBPs), members of the collectin family, have been implicated as lectin opsonins for various viruses and bacteria. Two distinct but related MBPs, MBP-A and MBP-C, with -55% identity at the amino acid level, have been previously characterized from rodents. In humans, however, only one form of MBP has been characterized. In this paper we report studies elucidating the evolution of primate MBPs. ELISA and Western blot analyses indicated that rhesus and cynomolgus monkeys have two forms of MBP in their sera, while chimpanzees have only one form, similar to humans. Two distinct MBP cDNA clones were isolated and characterized from a rhesus monkey liver cDNA library. Rhesus MBP-A is closely related to the mouse and rat MBP-A, showing 77% and 75% identity at the amino acid level, respectively. Rhesus MBP-A also has three cysteines at the N-terminus, similar to mouse and rat MBP-A and human MBP. Rhesus MBP-C shares 90% identity with the human MBP at the amino acid level and has three cysteines at the N-terminus, in contrast to two cysteine residues found in rodent MBP-C. A stretch of nine amino acids close to the N-terminus, absent in both mouse and rat MBP-A, but present in rodent MBP-C, chicken and human MBPs, is also found in the rhesus MBP-A. The phylogenetic analysis of rhesus and other mammalian MBPs, coupled with the serological data suggest that at least two distinct MBP genes existed prior to mammalian radiation and the hominoid ancestor apparently lost one of these genes or failed to express it.
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从人肝脏和血清中分离的甘露聚糖结合蛋白的结构和功能。
影响因子：
2.7
作者：
Hiroshi Kurata;T. Sannoh;Y. Kozutsumi;Yasunori Yokota;Toshisuke Kawasaki
通讯作者：
Toshisuke Kawasaki
人甘露糖结合蛋白是一种急性期反应物，与其他脊椎动物凝集素具有序列同源性[已发表的勘误表出现在 J Exp Med 1991 Sep 1;174(3):753]
影响因子：
15.3
作者：
By;B. Alan;Ezekowitz;E. Linda;Day;Gary;Herman
通讯作者：
Herman
甘露聚糖结合蛋白的五聚体/六聚体形式与酵母聚糖的结合可激活补体经典途径的酶原 C1r2C1s2 复合物，而无需 C1q 的参与。
影响因子：
4.4
作者：
Jinhua Lu;S. Thiel;H. Wiedemann;R. Timpl;K. Reid
通讯作者：
K. Reid
从大鼠血清中分离和表征两种不同的甘露聚糖结合蛋白。
影响因子：
3.9
作者：
S. Oka;Ken Ikeda;Toshisuke Kawasaki;Ikuo Yamashina
通讯作者：
Ikuo Yamashina
影响因子：
4.1
作者：
LU, JH;WILLIS, AC;REID, KBM
通讯作者：
REID, KBM