Basophil Activation to Gluten and Non-Gluten Proteins in Wheat-Dependent Exercise-Induced Anaphylaxis.
Basophil Activation to Gluten and Non-Gluten Proteins in Wheat-Dependent Exercise-Induced Anaphylaxis.
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DOI：
10.3389/falgy.2022.822554
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发表时间：
2022
影响因子：
--
通讯作者：
Scherf KA
中科院分区：
文献类型：
--
作者：
Gabler AM;Gebhard J;Norwig MC;Eberlein B;Biedermann T;Brockow K;Scherf KA
小麦依赖性运动过敏反应（WDEIA）是一种辅因子诱导的小麦过敏反应。谷蛋白，特别是ω5-麦醇溶蛋白，被认为是主要的过敏原，但部分水解的小麦蛋白（HWP）也发挥作用。我们的研究调查了面筋或HWP的分子组成和过敏性之间的联系。将谷蛋白（G）、ω5-麦醇溶蛋白含量降低的谷蛋白（G-ω5）、轻度处理的HWP（sHWP）和广泛处理的HWP（eHWP）的盐水提取物制备为过敏原试验溶液，并使用皮肤点刺试验和嗜碱性粒细胞活化试验（BAT）对12名WDEIA患者和10名对照进行过敏原性评估。补充十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）、高效液相色谱（HPLC）和质谱（MS）分析显示，G、G-ω5和sHWP过敏原试验溶液中主要为非谷蛋白，主要为α-淀粉酶/胰蛋白酶抑制剂（ATI）。只有eHWP含有醇溶蛋白和谷蛋白作为主要部分。与对照组相比，所有过敏原试验溶液均诱导患者中%CD63+嗜碱性粒细胞/抗Fc εRI比值显著升高。BAT使用sHWPs产生100%的灵敏度和83%的特异性在最佳截止值，并可能是有用的WDEIA诊断的另一种工具。我们的研究结果表明，非面筋蛋白携带尚未确定的过敏原表位似乎是相关的WDEIA。需要进一步的研究来阐明营养性ATI在WDEIA中的作用，并确定免疫激活的具体机制。
Wheat-dependent exercise-induced anaphylaxis (WDEIA) is a cofactor-induced wheat allergy. Gluten proteins, especially ω5-gliadins, are known as major allergens, but partially hydrolyzed wheat proteins (HWPs) also play a role. Our study investigated the link between the molecular composition of gluten or HWP and allergenicity. Saline extracts of gluten (G), gluten with reduced content of ω5-gliadins (G-ω5), slightly treated HWPs (sHWPs), and extensively treated HWPs (eHWPs) were prepared as allergen test solutions and their allergenicity assessed using the skin prick test and basophil activation test (BAT) on twelve patients with WDEIA and ten controls. Complementary sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), high-performance liquid chromatography (HPLC), and mass spectrometry (MS) analyses revealed that non-gluten proteins, mainly α-amylase/trypsin inhibitors (ATIs), were predominant in the allergen test solutions of G, G-ω5, and sHWPs. Only eHWPs contained gliadins and glutenins as major fraction. All allergen test solutions induced significantly higher %CD63+ basophils/anti-FcεRI ratios in patients compared with controls. BAT using sHWPs yielded 100% sensitivity and 83% specificity at optimal cut-off and may be useful as another tool in WDEIA diagnosis. Our findings indicate that non-gluten proteins carrying yet unidentified allergenic epitopes appear to be relevant in WDEIA. Further research is needed to clarify the role of nutritional ATIs in WDEIA and identify specific mechanisms of immune activation.
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影响因子：
3.8
作者：
Lagrain, Bert;Rombouts, Ine;Koehler, Peter
通讯作者：
Koehler, Peter
影响因子：
4.4
作者：
Gabler AM;Gebhard J;Eberlein B;Biedermann T;Scherf KA;Brockow K
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Brockow K
影响因子：
12.4
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影响因子：
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作者：
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