A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice.
A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice.
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DOI：
10.1038/nnano.2009.294
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发表时间：
2009-11
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
--
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半导体单壁碳纳米管固有的近红外光致发光是理想的生物成像，因为在超过1µm的近红外区域具有低自身荧光和深层组织穿透。然而，高量子产率的生物相容性单壁碳纳米管一直难以实现。在这里，我们展示了用胆酸钠对单壁碳纳米管进行超声处理，然后通过表面活性剂交换形成磷脂-聚乙二醇包被的纳米管，从而产生既明亮又具有生物相容性的体内显像剂。交换过程比直接用磷脂-聚乙二醇对纳米管进行超声处理要好，因为它对纳米管的破坏更小，并提高了量子产率。我们使用InGaAs相机在1 - 1.7µm光谱范围内，通过检测“交换”单壁碳纳米管在低剂量（17 mg l - 1注射剂量）下的固有近红外光致发光，展示了全动物体内成像。深层组织穿透和低自身荧光背景使得厚皮肤下肿瘤血管的高分辨率活体显微镜成像成为可能。
The near-infrared photoluminescence intrinsic to semiconducting single-walled carbon nanotubes is ideal for biological imaging owing to the low autofluorescence and deep tissue penetration in the near-infrared region beyond 1 µm. However, biocompatible single-walled carbon nanotubes with high quantum yield have been elusive. Here, we show that sonicating single-walled carbon nanotubes with sodium cholate, followed by surfactant exchange to form phospholipid–polyethylene glycol coated nanotubes, produces in vivo imaging agents that are both bright and biocompatible. The exchange procedure is better than directly sonicating the tubes with the phospholipid–polyethylene glycol, because it results in less damage to the nanotubes and improves the quantum yield. We show whole-animal in vivo imaging using an InGaAs camera in the 1–1.7 µm spectral range by detecting the intrinsic near-infrared photoluminescence of the ‘exchange’ single-walled carbon nanotubes at a low dose (17 mg l−1 injected dose). The deep tissue penetration and low autofluorescence background allowed high-resolution intravital microscopy imaging of tumour vessels beneath thick skin.
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影响因子：
56.9
作者：
O'Connell, MJ;Bachilo, SM;Smalley, RE
通讯作者：
Smalley, RE
影响因子：
10.8
作者：
Hertel, T;Hagen, A;Flahaut, E
通讯作者：
Flahaut, E
DOI：
10.1073/pnas.0707654105
发表时间：
2008-02-05
影响因子：
11.1
作者：
Liu, Zhuang;Davis, Corrine;Dai, Hongjie
通讯作者：
Dai, Hongjie
影响因子：
9.9
作者：
Liu, Zhuang;Tabakman, Scott;Welsher, Kevin;Dai, Hongjie
通讯作者：
Dai, Hongjie
影响因子：
10.8
作者：
Chiashi, Shohei;Watanabe, Satoshi;Homma, Yoshikazu
通讯作者：
Homma, Yoshikazu