The requirement of Zn2+ for the increase in ornithine decarboxylase induced by insulin and epidermal growth factor in primary cultured rat hepatocytes
The requirement of Zn2+ for the increase in ornithine decarboxylase induced by insulin and epidermal growth factor in primary cultured rat hepatocytes
复制标题
胰岛素和表皮生长因子诱导原代培养大鼠肝细胞鸟氨酸脱羧酶增加对Zn2的需求
DOI：
10.1016/s0955-2863(96)00060-5
复制
发表时间：
1996
影响因子：
5.6
通讯作者：
I. Matsui‐yuasa
中科院分区：
文献类型：
--
作者：
T. Nakatani;K. Ohtani;Y. Yano;S. Otani;I. Matsui‐yuasa
在原代培养的成年大鼠肝细胞上研究了限制性Zn ~（2+）对鸟氨酸脱羧酶（ODC）的影响。ODC活性增加4小时后加入胰岛素和表皮生长因子。最大活性维持12小时，然后下降，并返回到控制水平，由24小时。当螯合剂，二乙烯三胺五乙酸（DTPA）被添加到胰岛素和表皮生长因子，酶的活性下降了50%，只有锌2+的二价金属离子测试是有效的逆转这种影响。ODC信使RNA的水平不随螯合剂的加入而改变。螯合剂使ODC活性的半衰期降低2倍。DTPA暴露肝细胞可减少[3 H]胸苷掺入酸不溶性部分。加入600 μM Zn ~（2+）可完全逆转DNA合成的抑制作用。这些结果表明，肝细胞中缺乏Zn 2+损害ODC活性，主要是通过使酶不稳定，导致DNA合成的抑制。
The effect of restricted Zn2+on ornithine decarboxylase (ODC) was studied in primary cultured adult rat hepatocytes. ODC activity was increased 4 hr after adding insulin and epidermal growth factor. The maximal activity was maintained for 12 hr, it then decreased and returned to the control level by 24 hr. When the chelator, diethylenetriamine penta-acetic acid (DTPA) was added to insulin and epidermal growth factor, the enzyme activity was decreased by 50% and only the Zn2+of the bivalent metal ions tested was effective in reversing this effect. The level of the ODC messenger RNA did not change with addition of the chelator. The half-life of the ODC activity was decreased 2-fold by the chelator. Exposing the hepatocytes to DTPA reduced the incorporation of [3H]thymidine into acid insoluble fraction. The inhibition of DNA synthesis was fully reversed by the addition of 600 μM Zn2+. These results suggest that a lack of Zn2+in hepatocytes impairs ODC activity mainly by destabilizing the enzyme resulting in the inhibition of DNA synthesis.
人体锌代谢调节随年龄的变化。
DOI：
10.1152/ajpregu.1992.263.5.r1162
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Wastney,ME;Ahmed,S;Henkin,RI
通讯作者：
Henkin,RI
DOI：
10.1016/s0021-9258(18)83208-2
发表时间：
1989-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jeffrey B. Smith;Scott D. Dwyer;Lucinda Smith
通讯作者：
Jeffrey B. Smith;Scott D. Dwyer;Lucinda Smith