Expression of miR-200c corresponds with increased reactive oxygen species and hypoxia markers after transient focal ischemia in mice.
Expression of miR-200c corresponds with increased reactive oxygen species and hypoxia markers after transient focal ischemia in mice.
复制标题
DOI：
10.1016/j.neuint.2021.105146
复制
发表时间：
2021-10
影响因子：
4.2
通讯作者：
Stary CM
中科院分区：
文献类型：
--
作者：
Arvola O;Griffiths B;Rao A;Xu L;Pastroudis IA;Stary CM
栓塞性中风会导致注定死亡的细胞的坏死核心，也导致潜在可挽救的脑组织的围绕围周的分水岭区域。有效区分缺血性区域和缺血区域的方法对于新的治疗发现至关重要，以改善中风幸存者的预后。 MicroRNA是一类小型非编码RNA调节基因翻译，具有区域和细胞特异性表达以及对缺血的反应。我们先前已经报道说，在小鼠实验性中风后全球抑制脑microRNA-200C具有保护性，但是描述了势后区域和细胞类型的势头miR-200c表达的特定模式，以最大程度地减少攻击目标。效果和提前翻译应用。在这里，我们详细介绍了一种新的方案，可在实验性中风后可视化区域miR-200c表达，并与小鼠实验性卒中模型中的区域缺血和氧化应激的标记相吻合。在本研究中，我们证明了荧光缺氧指标Pimonidazole盐酸盐，反应性氧气标记物8-羟基 - 脱氧鸟苷，神经元标记MAP2和NEUN，Neun和Neun，以及反应性星形胶质细胞GFAP可以有效地复杂化的区域性差异。实验性中风后重新灌注后30分钟，并且可有效地将缺血性核心与周围的半体和未受影响的区域区分开，以进行靶向治疗。这种多维后击后免疫荧光成像方案可以更大程度地次区域机械研究，其最终目标是开发更有效的势后药物治疗。
Embolic stroke results in a necrotic core of cells destined to die, but also a peri-ischemic, watershed penumbral region of potentially salvageable brain tissue. Approaches to effectively differentiate between the ischemic and peri-ischemic zones is critical for novel therapeutic discovery to improve outcomes in survivors of stroke. MicroRNAs are a class of small non-coding RNAs regulating gene translation that have region- and cell-specific expression and responses to ischemia. We have previously reported that global inhibition of cerebral microRNA-200c after experimental stroke in mice is protective, however delineating the post-stroke sub-regional and cell-type specific patterns of post-stroke miR-200c expression are necessary to minimize off-target effects and advance translational application. Here, we detail a novel protocol to visualize regional miR-200c expression after experimental stroke, complexed with visualization of regional ischemia and markers of oxidative stress in an experimental stroke model in mice. In the present study we demonstrate that the fluorescent hypoxia indicator pimonidazole hydrochloride, the reactive-oxygen-species marker 8-hydroxy-deoxyguanosine, neuronal marker MAP2 and NeuN, and the reactive astrocyte marker GFAP can be effectively complexed to determine regional differences in ischemic injury as early as 30 mins post-reperfusion after experimental stroke, and can be effectively used to distinguish ischemic core from surrounding penumbral and unaffected regions for targeted therapy. This multi-dimensional post-stroke immunofluorescent imaging protocol enables a greater degree of sub-regional mechanistic investigation, with the ultimate goal of developing more effective post-stroke pharmaceutical therapy.
登录
查看更多内容
影响因子：
64.5
作者：
Goltsev Y;Samusik N;Kennedy-Darling J;Bhate S;Hale M;Vazquez G;Black S;Nolan GP
通讯作者：
Nolan GP
影响因子：
6.2
作者：
Buller, Benjamin;Chopp, Michael;Ueno, Yuji;Zhang, Li;Zhang, Rui Lan;Morris, Daniel;Zhang, Yi;Zhang, Zheng Gang
通讯作者：
Zhang, Zheng Gang
影响因子：
14.8
作者：
de Planell-Saguer, Mariangels;Celina Rodicio, Maria;Mourelatos, Zissimos
通讯作者：
Mourelatos, Zissimos
影响因子：
4.4
作者：
Guduric-Fuchs J;O'Connor A;Camp B;O'Neill CL;Medina RJ;Simpson DA
通讯作者：
Simpson DA
影响因子：
8.3
作者：
Lee, Soon-Tae;Chu, Kon;Roh, Jae-Kyu
通讯作者：
Roh, Jae-Kyu