Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging.
Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging.
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DOI：
10.1016/j.cell.2018.07.010
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发表时间：
2018-08-09
期刊：
影响因子：
64.5
通讯作者：
Nolan GP
中科院分区：
文献类型：
--
作者：
Goltsev Y;Samusik N;Kennedy-Darling J;Bhate S;Hale M;Vazquez G;Black S;Nolan GP
在此使用索引（法典）称为共同检测的高度多路复用的细胞仪成像方法，用于创建正常和狼疮的多路复用数据集（MRL/LPR）鼠Spleens。以及基于原位聚合的索引程序。在单细胞和细胞邻居水平上，覆盖了淋巴组织结构的众所周知的形态学特征，我们观察到细胞邻居对蛋白质受体在免疫细胞上的表达产生意外的，深远的影响。来自全身性自身免疫性疾病（MRL/LPR）的动物的脾观察到与解剖状态相比。
通过多路复用DNA标记的抗体染色（codex）分析自身免疫性
法典数据揭示了成对的相互作用，而壁ni与疾病发生了变化
邻居的第一层显着影响指数细胞中的标记表达
脾脏形态的变化与细胞频率的变化相关
基于DNA条形码的成像技术采用多重组织抗原染色来使细胞类型和动力学在自身免疫性疾病模型中的表征。
A highly multiplexed cytometric imaging approach, termed co-detection by indexing (CODEX), is used here to create multiplexed datasets of normal and lupus (MRL/lpr) murine spleens. CODEX iteratively visualizes antibody binding events using DNA barcodes, fluorescent dNTP analogs, and an in situ polymerization-based indexing procedure. An algorithmic pipeline for single-cell antigen quantification in tightly packed tissues was developed and used to overlay well-known morphological features with de novo characterization of lymphoid tissue architecture at a single-cell and cellular neighborhood levels. We observed an unexpected, profound impact of the cellular neighborhood on the expression of protein receptors on immune cells. By comparing normal murine spleen to spleens from animals with systemic autoimmune disease (MRL/lpr), extensive and previously uncharacterized splenic cell-interaction dynamics in the healthy versus diseased state was observed. The fidelity of multiplexed spatial cytometry demonstrated here allows for quantitative systemic characterization of tissue architecture in normal and clinically aberrant samples. Autoimmunity analyzed by multiplexed DNA-tagged antibody staining (CODEX) CODEX data reveal pairwise interactions and niches changing with disease First tier of neighbors significantly impacts marker expression in the index cells Changes in splenic morphology correlate with shifts in cell frequencies A DNA barcoding-based imaging technique uses multiplexed tissue antigen staining to enable the characterization of cell types and dynamics in a model of autoimmune disease.
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影响因子：
30.5
作者：
通讯作者：
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平铺 3D 显微图像采集的全局最佳拼接。
DOI：
10.1093/bioinformatics/btp184
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Preibisch S;Saalfeld S;Tomancak P
通讯作者：
Tomancak P
DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP
影响因子：
32.4
作者：
JACOBSON, BA;PANKA, DJ;MARSHAKROTHSTEIN, A
通讯作者：
MARSHAKROTHSTEIN, A
影响因子：
5.4
作者：
KOH, DR;HO, A;MAK, TW
通讯作者：
MAK, TW