Integrative Analysis of lncRNA and mRNA and Profiles in Postoperative Delirium Patients.
Integrative Analysis of lncRNA and mRNA and Profiles in Postoperative Delirium Patients.
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术后谵妄患者 lncRNA 和 mRNA 及概况的综合分析。
DOI：
10.3389/fnagi.2021.665935
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发表时间：
2021
影响因子：
4.8
通讯作者：
Mi W
中科院分区：
文献类型：
--
作者：
Song Y;Wang X;Hou A;Li H;Lou J;Liu Y;Cao J;Mi W
谵妄是大手术后常见的严重并发症。本研究的目的是探讨长链非编码rna （lncRNAs）和mrna在术后谵妄（POD）患者中的表达谱和功能网络。对4名POD患者和4名非POD志愿者的外周血样本进行微阵列分析，以鉴定差异表达（DE）的lncrna和mrna。通过定量反转录PCR （RT-qPCR）验证DE lncRNAs和mrna。我们进行了生物信息学分析，以确定POD的关键生物学功能和信号通路。在POD组和非POD组之间共鉴定出1195个DE lncrna和735个DE mrna。通过RT-qPCR验证，我们鉴定出14个可能与POD发病机制有关的DE lncrna。这14种DE lncrna在“谷氨酸和5-羟色胺”、“synaptotagmin 7”、“瞬时受体电位通道”、“白细胞介素-2的产生”中发挥重要的调节作用。lncRNA ENST00000530057与synaptotagmin (Syt) 7 mRNA存在调控关系。POD组PCLO mRNA水平上调。本研究显示POD中丰富的DE lncrna和mrna可能有助于破译疾病的发病机制。
Delirium is a common serious complication that often occurs after major surgery. The goals of this study were to explore the expression profiles and functional networks of long non-coding RNAs (lncRNAs) and mRNAs in patients of postoperative delirium (POD). Microarray analysis was performed on the peripheral blood samples to identify differentially expressed (DE) lncRNAs and mRNAs in 4 POD patients and 4 non-POD volunteers. DE lncRNAs and mRNAs were validated by quantitative reverse transcription PCR (RT-qPCR). Bioinformatic analyses were performed to identify the critical biological functions and signaling pathways involved in POD. A total of 1195 DE lncRNAs and 735 DE mRNAs were identified between the POD and non-POD groups. Verified by the RT-qPCR, we identified 14 DE lncRNAs that may relate to the pathogenesis of POD. These 14 DE lncRNAs play important regulatory roles in “glutamate and 5-hydroxytryptamine,” “synaptotagmin 7,” “transient receptor potential channel,” “interleukin-2 production.” There was a regulatory relationship between lncRNA ENST00000530057 and synaptotagmin (Syt) 7 mRNA. The mRNA level of PCLO was up-regulated in POD group. This study showed abundant DE lncRNAs and mRNAs in POD that might help in deciphering the disease pathogenesis.
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