Whole-Genome mRNA Gene Expression Differs Between Patients With and Without Delirium.
Whole-Genome mRNA Gene Expression Differs Between Patients With and Without Delirium.
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DOI：
10.1177/0891988718785774
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发表时间：
2018-07
影响因子：
2.6
通讯作者：
Douglas VC
中科院分区：
文献类型：
--
作者：
Kalantar K;LaHue SC;DeRisi JL;Sample HA;Contag CA;Josephson SA;Wilson MR;Douglas VC
旨在确定已知病因（尿路感染 [UTI]）的院内谵妄患者与未知病因的谵妄患者以及非谵妄患者之间基因表达的差异。这项前瞻性病例对照研究纳入了 30 名谵妄患者（8 名患有尿路感染）和 21 名非谵妄患者（11 名患有尿路感染）。对外周血信使 RNA 测序的转录组图谱进行了基因表达和疾病特异性途径富集模式的分析，从而纠正了全身炎症反应综合征。报告了基于 Fisher 精确检验（P < .05，|Zscore| >2）具有显着差异活性的基因和通路。与没有尿路感染的谵妄患者相比，患有尿路感染的谵妄患者表现出干扰素信号、上游细胞因子和转录调节因子的显着激活，以及肌动蛋白细胞骨架、整合素、桩蛋白、神经胶质瘤侵袭信号和上游生长因子的显着抑制。与非谵妄患者相比，所有谵妄患者的补体系统均显着激活。在没有 UTI 的谵妄患者中，与没有 UTI 的非谵妄患者相比，elF4 和 p7056 K 信号传导显着激活。由于尿路感染以及仅存在谵妄，谵妄患者之间的基因表达存在差异。转录谱分析可能有助于为谵妄患者开发病因特异性生物标志物。
To identify differences in gene expression between patients with in-hospital delirium from a known etiology (urinary tract infection [UTI]) and patients with delirium from an unknown etiology, as well as from nondelirious patients. Thirty patients with delirium (8 with UTI) and 21 nondelirious patients (11 with UTI) were included in this prospective case–control study. Transcriptomic profiles from messenger RNA sequencing of peripheral blood were analyzed for gene expression and disease-specific pathway enrichment patterns, correcting for systemic inflammatory response syndrome. Genes and pathways with significant differential activity based on Fisher exact test (P < .05, |Zscore| >2) are reported. Patients with delirium with UTI, compared to patients with delirium without UTI, exhibited significant activation of interferon signaling, upstream cytokines, and transcription regulators, as well as significant inhibition of actin cytoskeleton, integrin, paxillin, glioma invasiveness signaling, and upstream growth factors. All patients with delirium, compared to nondelirious patients, had significant complement system activation. Among patients with delirium without UTI, compared to nondelirious patients without UTI, there was significant activation of elF4 and p7056 K signaling. Differences exist in gene expression between delirious patients due to UTI presence, as well as due to the presence of delirium alone. Transcriptional profiling may help develop etiology-specific biomarkers for patients with delirium.
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DOI：
10.1016/j.athoracsur.2015.12.074
发表时间：
2016-05
期刊：
The Annals of thoracic surgery
影响因子：
--
作者：
Brown CH 4th;Laflam A;Max L;Lymar D;Neufeld KJ;Tian J;Shah AS;Whitman GJ;Hogue CW
通讯作者：
Hogue CW
影响因子：
5.2
作者：
Capri M;Yani SL;Chattat R;Fortuna D;Bucci L;Lanzarini C;Morsiani C;Catena F;Ansaloni L;Adversi M;Melotti MR;Di Nino G;Franceschi C
通讯作者：
Franceschi C
影响因子：
168.9
作者：
Inouye, Sharon K.;Westendorp, Rudi G. J.;Saczynski, Jane S.
通讯作者：
Saczynski, Jane S.
影响因子：
--
作者：
Inouye, Sharon K.;Zhang, Ying;Marcantonio, Edward R.
通讯作者：
Marcantonio, Edward R.
影响因子：
120.7
作者：
FRANCIS, J;MARTIN, D;KAPOOR, WN
通讯作者：
KAPOOR, WN