Targeting of Salmonella typhimurium to vesicles containing lysosomal membrane glycoproteins bypasses compartments with mannose 6-phosphate receptors.
Targeting of Salmonella typhimurium to vesicles containing lysosomal membrane glycoproteins bypasses compartments with mannose 6-phosphate receptors.
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将鼠伤寒沙门氏菌靶向含有溶酶体膜糖蛋白的囊泡，绕过带有甘露糖6-磷酸受体的室。
DOI：
10.1083/jcb.129.1.81
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发表时间：
1995-04
影响因子：
7.8
通讯作者：
FINLAY, BB
中科院分区：
文献类型：
--
作者：
GARCIADELPORTILLO, F;FINLAY, BB
鼠伤寒沙门氏菌是一种细胞内细菌病原体，进入宿主细胞后仍封闭在空泡（SCV）中。在这项研究中，我们研究了S的细胞内运输路线。上皮细胞内的鼠伤寒。间接免疫荧光分析表明，细菌开始融合与溶酶体膜糖蛋白（lgp）含有车厢约15分钟后细菌内化。这个过程在大约75分钟后完成，并且不需要微管。在SCV中未观察到可检测水平的阳离子非依赖性（CI）或阳离子依赖性（CD）甘露糖6-磷酸受体（M6 PR）。溶酶体酶在SCV中表现出不同的分布：溶酶体酸性磷酸酶（ALP）以与LGPS相同的动力学掺入这些空泡中，而组织蛋白酶D在SCV中的比例较低（约30%）。用流体内吞示踪剂如荧光素-硫酸葡聚糖（F-DX）或辣根过氧化物酶（HRP）的摄取实验显示，在摄取2小时后，F-DX存在于未感染细胞中约75%的含lgp囊泡中，而在相同的摄取期间，仅约15%的SCV含有少量的示踪剂。SCV也显示只有部分融合HRP预装的次级溶酶体，约30%的SCV具有可检测量的HRP在感染后6小时。这些结果表明，SCV显示有限的可及性流体内吞示踪剂和成熟的溶酶体，因此，功能上分离的内吞途径。此外，S.上皮细胞内的鼠伤寒沙门氏菌的研究使我们能够确定在沙门氏菌感染的细胞中存在两种不同的含有lgp的囊泡：一种群体从内吞途径分离，与进入的SCV融合，并且可能由分泌途径产生，而第二种涉及经典的次级或成熟溶酶体。
Salmonella typhimurium is an intracellular bacterial pathogen that remains enclosed in vacuoles (SCV) upon entry into the host cell. In this study we have examined the intracellular trafficking route of S. typhimurium within epithelial cells. Indirect immunofluorescence analysis showed that bacteria initiated fusion with lysosomal membrane glycoprotein (lgp)-containing compartments approximately 15 min after bacterial internalization. This process was completed approximately 75 min later and did not require microtubules. Cation-independent (CI)- or cation-dependent (CD)-mannose 6-phosphate receptors (M6PRs) were not observed at detectable levels in SCV. Lysosomal enzymes showed a different distribution in SCV: lysosomal-acid phosphatase (LAP) was incorporated into these vacuoles with the same kinetics as lgps, while cathepsin D was present in a low proportion (approximately 30%) of SCV. Uptake experiments with fluid endocytic tracers such as fluorescein- dextran sulphate (F-DX) or horseradish-peroxidase (HRP) showed that after 2 h of uptake, F-DX was present in approximately 75% of lgp- containing vesicles in uninfected cells, while only approximately 15% of SCV contained small amounts of the tracer during the same uptake period. SCV also showed only partial fusion with HRP-preloaded secondary lysosomes, with approximately 30% of SCV having detectable amounts of HRP at 6 h after infection. These results indicate that SCV show limited accessibility to fluid endocytic tracers and mature lysosomes, and are therefore functionally separated from the endocytic route. Moreover, the unusual intracellular trafficking route of S. typhimurium inside epithelial cells has allowed us to establish the existence of two different lgp-containing vesicles in Salmonella- infected cells: one population is separated from the endocytic route, fusogenic with incoming SCV and may arise from a secretory pathway, while the second involves the classical secondary or mature lysosomes.
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DOI：
10.1083/jcb.124.5.677
发表时间：
1994-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Desjardins M;Huber LA;Parton RG;Griffiths G
通讯作者：
Griffiths G
影响因子：
2.7
作者：
FURUNO, K;YANO, S;KATO, K
通讯作者：
KATO, K
内吞囊泡中的甘露糖6-磷酸受体和溶酶体膜蛋白的分选。
影响因子：
7.8
作者：
Geuze, H J;Stoorvogel, W;Strous, G J;Slot, J W;Bleekemolen, J E;Mellman, I
通讯作者：
Mellman, I
影响因子：
11.4
作者：
BRAUN, M;WAHEED, A;VONFIGURA, K
通讯作者：
VONFIGURA, K
影响因子：
3.6
作者：
GARCIADELPORTILLO, F;FOSTER, JW;FINLAY, BB
通讯作者：
FINLAY, BB