TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA-targeting proteins.
TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA-targeting proteins.
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DOI：
10.1016/j.tcb.2013.04.003
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发表时间：
2013-08
影响因子：
19
通讯作者：
Bogdanove, Adam J.
中科院分区：
文献类型：
--
作者：
Doyle, Erin L.;Stoddard, Barry L.;Voytas, Daniel F.;Bogdanove, Adam J.
关键词：
转录激活因子类(TAL)效应物是由黄单胞菌属病原菌注入植物细胞的转录因子。它们通过激活对疾病重要的宿主基因来发挥毒力因子的作用，或者通过启动提供抗性的基因来作为无毒因子发挥作用。DNA结合特异性是由每个蛋白质中与不同核苷酸一一对应的多态重复序列编码的。这种编码促进了目标识别，并为工程抗病开辟了新的途径。它还实现了TAL效应器的定制，用于靶向基因控制、基因组编辑和其他应用。本文综述了TAL效应的结构基础-DNA专一性，TAL效应-DNA编码对植物病理学和工程抗性的影响，以及基于TAL效应的DNA靶向的最新成就和未来挑战。
Transcription activator-like (TAL) effectors are transcription factors injected into plant cells by pathogenic bacteria in the genus Xanthomonas. They function as virulence factors by activating host genes important for disease, or as avirulence factors by turning on genes that provide resistance. DNA binding specificity is encoded by polymorphic repeats in each protein that correspond one-to-one with different nucleotides. This code has facilitated target identification and opened new avenues for engineering disease resistance. It has also enabled TAL effector customization for targeted gene control, genome editing, and other applications. This article reviews the structural basis for TAL effector-DNA specificity, the impact of the TAL effector-DNA code on plant pathology and engineered resistance, and recent accomplishments and future challenges in TAL effector-based DNA targeting.
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