Molecular and Functional Characterization of NKG2D, NKp80, and NKG2C Triggering NK Cell Receptors in Rhesus and Cynomolgus Macaques: Monitoring of NK Cell Function during Simian HIV Infection1
Molecular and Functional Characterization of NKG2D, NKp80, and NKG2C Triggering NK Cell Receptors in Rhesus and Cynomolgus Macaques: Monitoring of NK Cell Function during Simian HIV Infection1
复制标题
恒河猴和食蟹猴中 NKG2D、NKp80 和 NKG2C 触发 NK 细胞受体的分子和功能表征：猴 HIV 感染期间 NK 细胞功能的监测1
作者：
R. Biassoni;M. Fogli;C. Cantoni;P. Costa;R. Conte;G. Koopman;A. Cafaro;B. Ensoli;A. Moretta;L. Moretta;A. De Maria
最近，在正常和疾病条件下，如HIV感染和急性髓系白血病，先天免疫和NK细胞参与了适应性免疫反应的启动。到目前为止，对NK细胞触发受体表达的分析仅限于NKp46和NKp30在猕猴中的表达。在这项研究中，我们将分子和功能的表征扩展到使用PBMC的各种NK细胞触发受体，并通过细胞荧光法和细胞杀伤活性分析扩展到体外来源的NK细胞群体。此外，本研究还利用RT-PCR技术、克隆测序和瞬时转基因等方法对束支原体和猕猴的NKp80、NKG2D、CD94/NKG2C和CD94/NKG2A以及信号转导多肽DNAX激活蛋白DAP-10进行了鉴定和鉴定。束支原体和木霉NK细胞均表达NKp80、NKG2D和NKG2C分子，与人类NK细胞具有高度的序列同源性。对感染HIV的猿猴的NK细胞的分析显示，仅在某些动物中，特定的NK细胞触发受体的表面表达减少与NK细胞功能下降有关。与未感染动物相比，体外培养的小鼠外周血细胞上NK细胞激活受体的表面密度及其对NK细胞群的激活功能总体上并未降低。因此，触发对猕猴NK细胞的NK细胞受体监测是可能的，并可能为评估实验感染期间的NK细胞功能以及探索人类与不同接种策略的食蟹猴和恒河猴之间的免疫保护相关性的可能差异提供有价值的工具。
An involvement of innate immunity and of NK cells during the priming of adaptive immune responses has been recently suggested in normal and disease conditions such as HIV infection and acute myelogenous leukemia. The analysis of NK cell-triggering receptor expression has been so far restricted to only NKp46 and NKp30 in Macaca fascicularis. In this study, we extended the molecular and functional characterization to the various NK cell-triggering receptors using PBMC and to the in vitro-derived NK cell populations by cytofluorometry and by cytolytic activity assays. In addition, RT-PCR strategy, cDNA cloning/sequencing, and transient transfections were used to identify and characterize NKp80, NKG2D, CD94/NKG2C, and CD94/NKG2A in M. fascicularis and Macaca mulatta as well as in the signal transducing polypeptide DNAX-activating protein DAP-10. Both M. fascicularis and M. mulatta NK cells express NKp80, NKG2D, and NKG2C molecules, which displayed a high degree of sequence homology with their human counterpart. Analysis of NK cells in simian HIV-infected M. fascicularis revealed reduced surface expression of selected NK cell-triggering receptors associated with a decreased NK cell function only in some animals. Overall surface density of NK cell-triggering receptors on peripheral blood cells and their triggering function on NK cell populations derived in vitro was not decreased compared with uninfected animals. Thus, triggering NK cell receptor monitoring on macaque NK cells is possible and could provide a valuable tool for assessing NK cell function during experimental infections and for exploring possible differences in immune correlates of protection in humans compared with cynomolgus and rhesus macaques undergoing different vaccination strategies.
用于艾滋病毒疫苗开发的非人类灵长类动物模型。
DOI：
--
发表时间：
1992
期刊：
Immunodeficiency reviews
影响因子：
--
作者：
Letvin,NL
通讯作者：
Letvin,NL
在中和抗体存在的情况下注射 MV DNA 疫苗，可保护幼年猕猴免受麻疹病毒 (MV) 的攻击。
DOI：
10.1086/420792
发表时间：
2004
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Premenko-Lanier,Mary;Rota,PaulA;Rhodes,GaryH;Bellini,WilliamJ;McChesney,MichaelB
通讯作者：
McChesney,MichaelB
非人类灵长类动物对同种异体移植物耐受的免疫决定需要早期抑制先天免疫并诱导免疫调节。
影响因子：
1.5
作者：
Hutchings,Anne;Wu,Jianguo;Asiedu,Clement;Hubbard,William;Eckhoff,Devin;Contreras,Juan;Thomas,FrancisT;Neville,David;Thomas,JudithM
通讯作者：
Thomas,JudithM