New platform technology for comprehensive serological diagnostics of autoimmune diseases.
New platform technology for comprehensive serological diagnostics of autoimmune diseases.
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DOI：
10.1155/2012/284740
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发表时间：
2012
影响因子：
--
通讯作者：
Roggenbuck D
中科院分区：
文献类型：
--
作者：
Willitzki A;Hiemann R;Peters V;Sack U;Schierack P;Rödiger S;Anderer U;Conrad K;Bogdanos DP;Reinhold D;Roggenbuck D
抗体检测是自身免疫性疾病血清学诊断的重要环节。然而，对于血清，特别是系统性自身免疫性风湿病(SAD)患者的血清，已经提出了不同的诊断策略。一般来说，通过间接免疫荧光(IIF)筛选SARD相关抗体之后，再进行涵盖不同检测技术的确证测试。由于IIF筛查缺乏自动化、标准化、现代数据管理和人为偏见，这种两阶段方法最近受到多重技术的挑战，特别是在工作量较大的实验室。然而，IIF检测抗核抗体仍被推荐为筛选疑似SARD患者血清抗体的金标准方法。为了解决IIF的局限性和满足低成本的自身抗体筛选的需求，最近引入了利用新的模式识别算法进行图像分析的自动化IIF方法。在这方面，AKLIDES技术已经成为第一个商业化的基于细胞的IIF检测的自动解释平台，并通过可寻址微珠免疫分析提供多路复用，用于验证性测试。本文概述了最近发表的证明这项新技术在SARD血清学中的优势的研究。
Antibody assessment is an essential part in the serological diagnosis of autoimmune diseases. However, different diagnostic strategies have been proposed for the work up of sera in particular from patients with systemic autoimmune rheumatic disease (SARD). In general, screening for SARD-associated antibodies by indirect immunofluorescence (IIF) is followed by confirmatory testing covering different assay techniques. Due to lacking automation, standardization, modern data management, and human bias in IIF screening, this two-stage approach has recently been challenged by multiplex techniques particularly in laboratories with high workload. However, detection of antinuclear antibodies by IIF is still recommended to be the gold standard method for antibody screening in sera from patients with suspected SARD. To address the limitations of IIF and to meet the demand for cost-efficient autoantibody screening, automated IIF methods employing novel pattern recognition algorithms for image analysis have been introduced recently. In this respect, the AKLIDES technology has been the first commercially available platform for automated interpretation of cell-based IIF testing and provides multiplexing by addressable microbead immunoassays for confirmatory testing. This paper gives an overview of recently published studies demonstrating the advantages of this new technology for SARD serology.
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影响因子：
3.5
作者：
Copple, Susan S.;Sawitzke, Allen D.;Hill, Harry R.
通讯作者：
Hill, Harry R.
DOI：
10.1196/annals.1398.046
发表时间：
2007-01-01
期刊：
AUTOIMMUNITY, PART A: BASIC PRINCIPLES AND NEW DIAGNOSTIC TOOLS
影响因子：
--
作者：
Ghillani, P.;Rouquette, A. M.;Musset, L.
通讯作者：
Musset, L.
影响因子：
5
作者：
González, C;García-Berrocal, B;González-Buitrago, JM
通讯作者：
González-Buitrago, JM
影响因子：
--
作者：
Hiemann, Rico;Roggenbuck, Dirk;Conrad, Karsten
通讯作者：
Conrad, Karsten
影响因子：
13.6
作者：
Hiemann, Rico;Buettner, Thomas;Conrad, Karsten
通讯作者：
Conrad, Karsten