Marking Fish with Fluorochrome Dyes

Marking Fish with Fluorochrome Dyes
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用荧光染料标记鱼

DOI:
10.1080/23308249.2019.1681358
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发表时间:
2020-01
影响因子:
11.5
通讯作者:
Weizhi Yao
Weizhi Yao
中科院分区:
农林科学1区
文献类型:
--
作者:
Hongjian Lü;Mei Fu;Zhixin Zhang;Shengqi Su;Weizhi Yao

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摘要利用荧光染料对鱼类进行标记,可以在耳石、鳞片、鳍线、鳍刺和其他骨骼结构上产生可检测的标记,这是一种广泛应用于包括硬骨鱼和棘鱼在内的鱼类的大规模标记的技术。这项技术具有相对经济的优势和简单的标记过程,可以应用于所有年龄的鱼类,这些荧光标记可以保留几个月甚至几年。到目前为止,该标记技术已经成功地标记了200种鱼类。荧光标记是规模化标记孵化养殖鱼类的一种实用有效的标记技术。综述了荧光标记的方法学,包括荧光标记染料、应用方法、检测方法和评价策略。还讨论了荧光标记的实际应用、限制和监督方面的问题。
Abstract Marking fish with fluorochrome dyes has the potential to produce detectable marks in otoliths, scales, fin rays, fin spines, and other bony structures based on differential staining of chemical dyes, and it is a widely used technique for mass-marking fish that included both teleosts and elasmobranchs. This technique, which has relatively economic advantages and simple marking process, can be applied to fish of all ages, and these fluorescent marks can be retained for several months and even years. Up to now, >200 fish species have been successfully marked by this marking technique. Fluorescent labeling is a practical and available marking technique for large-scale marking hatchery-reared fish. The methodology of fluorescent labeling is reviewed, including fluorochrome labeling dyes, application method, detection, and evaluation strategies. Aspects of practical applications, limitations, and supervision of fluorescent labeling are also discussed.
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