Alginate encapsulated multipotent adult progenitor cells promote corneal stromal cell activation via release of soluble factors.
Alginate encapsulated multipotent adult progenitor cells promote corneal stromal cell activation via release of soluble factors.
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DOI：
10.1371/journal.pone.0202118
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Connon CJ
中科院分区：
文献类型：
--
作者：
Al-Jaibaji O;Swioklo S;Gijbels K;Vaes B;Figueiredo FC;Connon CJ
为了减少日益增长的角膜移植需求，目前正在尝试恢复角膜透明度，一种有效的细胞来源是多能成体祖细胞 (MAPC®)。这些细胞释放出强大的旁分泌因子混合物，可以指导伤口愈合和组织再生。然而，它们在角膜再生中的作用却被忽视了。因此，我们试图探索将海藻酸盐的细胞保护储存特性与 MAPC 相结合以生成可储存的充满细胞的凝胶用于角膜伤口愈合的潜力。低温储存 72 小时后，海藻酸盐封装可在 4 或 15°C 下保持 MAPC 活力。封装的 MAPC（2 x106 细胞/mL）储存在 15°C 下，是细胞恢复的最佳温度。这些细胞能够重新附着到组织培养塑料上，同时表现出正常的表型，并且这保持在无血清和无异生素的培养基中。此外，与无细胞对照相比，在海藻酸盐封装的 MAPC 存在下，角膜基质细胞的划痕显着减少（p = 0.018）。这项研究表明，将 MAPC 固定在藻酸盐水凝胶内不会妨碍它们通过可溶性因子影响次级细胞群的能力，并且这些效应在低温储存后成功保留。
To reduce the increasing need for corneal transplantation, attempts are currently aiming to restore corneal clarity, one potent source of cells are multipotent adult progenitor cells (MAPC®). These cells release a powerful cocktail of paracrine factors that can guide wound healing and tissue regeneration. However, their role in corneal regeneration has been overlooked. Thus, we sought to explore the potential of combining the cytoprotective storage feature of alginate, with MAPC to generate a storable cell-laden gel for corneal wound healing. 72 hours following hypothermic storage, alginate encapsulation was shown to maintain MAPC viability at either 4 or 15°C. Encapsulated MAPC (2 x106 cells/mL) stored at 15°C presented the optimum temperature that allowed for cell recovery. These cells had the ability to reattach to tissue culture plastic whilst exhibiting normal phenotype and this was maintained in serum-free and xenobiotic-free medium. Furthermore, corneal stromal cells presented a significant decrease in scratch-wounds in the presence of alginate encapsulated MAPC compared to a no-cell control (p = 0.018). This study shows that immobilization of MAPC within an alginate hydrogel does not hinder their ability to affect a secondary cell population via soluble factors and that these effects are successfully retained following hypothermic storage.
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