Logic-embedded vectors for intracellular partitioning, endosomal escape, and exocytosis of nanoparticles.
Logic-embedded vectors for intracellular partitioning, endosomal escape, and exocytosis of nanoparticles.
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DOI：
10.1002/smll.201000727
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发表时间：
2010-12-06
期刊：
影响因子：
13.3
通讯作者：
Ferrari, Mauro
中科院分区：
文献类型：
--
作者：
Serda, Rita E.;Mack, Aaron;van de Ven, Anne L.;Ferrati, Silvia;Dunner, Kenneth, Jr.;Godin, Biana;Chiappini, Ciro;Landry, Matthew;Brousseau, Louis;Liu, Xuewu;Bean, Andrew J.;Ferrari, Mauro
新一代的纳米载体，逻辑嵌入载体（LEV），被赋予了在多个细胞内位点定位组件的能力，创造了协同控制冗余或双重打击途径的机会。LEV编码元件包括大小、形状、电荷和表面化学。在这项研究中，LEV由多孔硅纳米载体和表面定制的氧化铁纳米颗粒组成，多孔硅纳米载体被编程为沿着内体途径进行细胞摄取和运输，表面定制的氧化铁纳米颗粒被编程为内体分选和将颗粒分配到独特的细胞位置。在硅纳米载体的持续内体定位的存在下，胺官能化的纳米颗粒被分选成多个囊泡体，其形成与细胞分泌相容的新型膜结合隔室，而壳聚糖涂覆的纳米颗粒从内体逃逸并进入胞质溶胶。多孔硅基质内的封装保护这些纳米颗粒表面定制的性质，增强壳聚糖涂覆的纳米颗粒的内体逃逸。因此，LEV提供了一种机制，用于将纳米颗粒屏蔽运输到病变，在多个水平上进行细胞操纵，以及用于靶向细胞内和细胞间的手段。
A new generation of nanocarriers, logic-embedded vectors (LEVs), is endowed with the ability to localize components at multiple intracellular sites, creating an opportunity for synergistic control of redundant or dual-hit pathways. LEV encoding elements include size, shape, charge, and surface chemistry. In this study, LEVs consist of porous silicon nanocarriers, programmed for cellular uptake and trafficking along the endosomal pathway, and surface-tailored iron oxide nanoparticles, programmed for endosomal sorting and partitioning of particles into unique cellular locations. In the presence of persistent endosomal localization of silicon nanocarriers, amine-functionalized nanoparticles are sorted into multiple vesicular bodies that form novel membrane-bound compartments compatible with cellular secretion, while chitosan-coated nanoparticles escape from endosomes and enter the cytosol. Encapsulation within the porous silicon matrix protects these nanoparticle surface tailored-properties, enhancing endosomal escape of chitosan coated nanoparticles. Thus LEVs provide a mechanism for shielded transport of nanoparticles to the lesion, cellular manipulation at multiple levels, and a means for targeting both within and between cells.
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