Effect of filtrate flux and process disruptions on virus retention by a relatively homogeneous virus removal membrane
Effect of filtrate flux and process disruptions on virus retention by a relatively homogeneous virus removal membrane
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滤液通量和过程中断对相对均匀的病毒去除膜截留病毒的影响
DOI：
10.1002/btpr.3255
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发表时间：
2022
影响因子：
2.9
通讯作者：
Zydney, Andrew L.
中科院分区：
文献类型：
--
作者：
Afzal, Mohammad A.;Zydney, Andrew L.
最近的研究表明，在低流速和工艺中断后，特定病毒过滤器的病毒截留率可以降低;然而，病毒截留率的这些变化的幅度和控制这种行为的潜在机制仍然没有得到很好的理解。本研究的目的是定量了解控制相对均匀的聚偏氟乙烯病毒去除过滤器的病毒截留行为的因素。数据是用噬菌体E174作为模型病毒获得的。病毒截留率随着滤液通量的降低而降低，并且在病毒过滤过程中也略有下降。根据中断的持续时间和时间，工艺中断后病毒保留立即减少多达1000倍（3个对数）。使用内部极化模型充分描述了实验结果，该模型解释了过滤/破坏过程中病毒的积累和释放，关键模型参数取决于滤液通量。这些结果为控制病毒保留行为的因素提供了重要的见解，并为在生物加工中有效使用病毒去除过滤器提供了指导。
Recent studies have shown that virus retention by specific virus filters can be reduced at low flow rates and after process disruptions; however, the magnitude of these changes in virus retention and the underlying mechanisms controlling this behavior are still not well understood. The objective of this study was to develop a quantitative understanding of the factors controlling the virus retention behavior of a relatively homogeneous polyvinylidene fluoride virus removal filter. Data were obtained with the bacteriophage ϕX174 as a model virus. Virus retention decreased as the filtrate flux was reduced and also declined slightly over the course of the virus filtration. Virus retention immediately after a process disruption decreased by as much as a factor of 1000 (3‐logs) depending on the duration and timing of the disruption. The experimental results were well‐described using an internal polarization model that accounts for accumulation and release of virus during the filtration / disruption, with the key model parameters dependent on the filtrate flux. These results provide important insights into the factors controlling the virus retention behavior as well as guidelines for the effective use of virus removal filters in bioprocessing.
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